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| Status |
Public on Mar 22, 2022 |
| Title |
Relish-mediated lncRNA-CR33942 facilitates Drosophila Imd immune responses and promotes antimicrobial peptide expression via interacting with Relish |
| Organism |
Drosophila melanogaster |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Long noncoding RNAs (lncRNAs), as a class of emerging regulators, play crucial role in regulating the strength and duration of innate immunity. However, little is known about how these Drosophila Imd immunity-related lncRNAs are regulated. Herein, we firstly revealed that overexpression of lncRNA-CR33942 could strengthen the expression of Imd pathway antimicrobial peptides Diptericin (Dpt) and Attacin-A (AttA) after infection, and vice versa. Secondly, RNA-seq analysis of post-infected lncRNA-CR33942-overexpressing flies further confirmed that lncRNA-CR33942 positively regulates the Drosophila Imd pathway. Mechanistically, we indicated that lncRNA-CR33942 interacts with NF-κB transcription factor Relish to promote its binding to Dpt and AttA promoters, thereby facilitating Dpt and AttA expression. Interestingly, we found that Relish can also directly promote lncRNA-CR33942 transcription by binding to its promoter. Finally, rescue experiments and dynamic expression profiling post-infection demonstrated the vital role of the Relish/lncRNA-CR33942/AMPs regulatory axis in enhancing inadequate Imd immune responses and maintaining immune homeostasis. Taken together, our study not only elucidates a novel mechanism about lncRNA in Drosophila Imd immune regulation, but also has important guiding significance for elucidating the complex regulatory mechanism of animal innate immune response.
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| Overall design |
the CR33942-overexpressing and control male adult flies were collected at 12 h after E. coli infection for subsequently RNA-seq
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| Contributor(s) |
Zhou H, Li S, Ma F, Jin P |
| Citation(s) |
35720331 |
| |
| Submission date |
Mar 19, 2022 |
| Last update date |
Jun 27, 2022 |
| Contact name |
Hongjian Zhou |
| E-mail(s) |
zhouhongjian8189@outlook.com
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| Phone |
18852098189
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| Organization name |
Nanjing Normal University
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| Street address |
No.1 Wenyuan Road, Xianlin University Town, Nanjing
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| City |
Nanjing |
| ZIP/Postal code |
210023 |
| Country |
China |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA817795 |
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