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| Status |
Public on May 19, 2023 |
| Title |
Tissue-intrinsic beta-catenin signals antagonize Nodal-driven anterior visceral endoderm differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The anterior-posterior axis of the mammalian embryo is laid down by the anterior visceral endoderm (AVE), an extraembryonic signaling center that is specified within the visceral endoderm. Current models posit that AVE differentiation is promoted globally by epiblast-derived Nodal signals, and spatially restricted by a BMP gradient established by the extraembryonic ectoderm. Here, we report spatially restricted AVE differentiation in bilayered embryo-like aggregates made from mouse embryonic stem cells that lack an extraembryonic ectoderm. Notably, clusters of AVE cells also form in pure visceral endoderm cultures upon activation of Nodal signaling, indicating that tissue-intrinsic factors can restrict AVE differentiation. We identify β-catenin activity as a tissue-intrinsic factor that antagonizes AVE-inducing Nodal signals. Together, our results show how an AVE-like population can arise through interactions between epiblast and visceral endoderm alone. This mechanism may be a flexible solution for axis patterning in a wide range of embryo geometries, and provide robustness to axis patterning when coupled with signal gradients.
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| Overall design |
We performed single cell RNA sequencing on cells obtained from three types of multicellular aggregates: bilayered embryo-like aggregates (BELAs), VE cysts and Epi cysts.
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| Web link |
https://www.nature.com/articles/s41467-024-49380-0
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| Contributor(s) |
Schumacher S, Fernkorn M, Marten M, Schröter C |
| Citation(s) |
38871742 |
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| Submission date |
Mar 16, 2022 |
| Last update date |
Jun 14, 2024 |
| Contact name |
Christian Schröter |
| E-mail(s) |
christian.schroeter@mpi-dortmund.mpg.de
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| Phone |
+492311332223
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| Organization name |
MPI of Molecular Physiology
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| Department |
Systemic Cell Biology
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| Street address |
Otto-Hahn-Str. 11
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| City |
Dortmund |
| State/province |
Nordrhein-Westfalen |
| ZIP/Postal code |
44227 |
| Country |
Germany |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA816861 |
