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| Status |
Public on Mar 07, 2022 |
| Title |
Comparative bulk RNAseq analysis of CXCL12-abundant retiucular (CAR) cells, fetal perichondrium-derived CAR cells and chondrocyte-derived CAR cells. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We revealed the unique molecular signature characterizing fetal perichondrium-derived and chondrocyte-derived CAR cells
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| |
|
| Overall design |
GFP+tdTomato+ cells were FACS-isolated from postnatal day 21 (P21) femurs of Cxcl12-GFP; Dlx5-creER; R26R-tdTomato and Cxcl12-GFP; Fgfr3-iCreER; R26R-tdTomato mice, both pulsed with a single dose of tamoxifen at embryonic day 12.5 (E12.5). mRNA were amplifed by SMARTer mRNA-seq kit (Clonetech/Takara) before deep-sequenced by Illumina HiSeq4000.
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| |
|
| Contributor(s) |
Ono N |
| Citation(s) |
36443296 |
| |
| Submission date |
Mar 04, 2022 |
| Last update date |
Dec 15, 2022 |
| Contact name |
Noriaki Ono |
| E-mail(s) |
noriaki.ono@uth.tmc.edu
|
| Phone |
7134860539
|
| Organization name |
University of Texas Health Science Center at Houston
|
| Department |
School of Dentistry
|
| Lab |
Ono lab
|
| Street address |
1941 East Rd, Rm 5322
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77054 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (5)
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|
| Relations |
| BioProject |
PRJNA812900 |
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