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| Status |
Public on Mar 06, 2022 |
| Title |
Phototoxic effects of nonlinear optical microscopy on cell cycle, oxidative states, and gene expression |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Nonlinear optical imaging modalities, such as stimulated Raman scattering (SRS) microscopy, use pulsed-laser excitation with high peak intensity that can perturb the native state of cells. In this study, we used bulk RNA sequencing, quantitative measurement of cell proliferation, and fluorescent measurement of the generation of reactive oxygen species (ROS) to assess phototoxic effects of near-IR pulsed laser radiation, at different time scales, for laser excitation settings relevant to SRS imaging. We define a range of laser excitation settings for which there was no significant change in mouse Neuro2A cells after laser exposure. This study provides guidance for imaging parameters that minimize photo-induced perturbations in SRS microscopy to ensure accurate interpretation of experiments with time-lapse imaging or with paired measurements of imaging and sequencing on the same cells.
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| Overall design |
Bulk RNA-sequencing of mouse neuroblastoma (N2A) cells with and without laser irradiation. Three replicates were sequenced for each condition.
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| Contributor(s) |
Zhang X, Dorlhiac G, Landry MP, Streets A |
| Citation(s) |
36335145 |
| |
| Submission date |
Mar 01, 2022 |
| Last update date |
Nov 29, 2022 |
| Contact name |
Xinyi Zhang |
| E-mail(s) |
xinyizxx@mit.edu
|
| Organization name |
UC Berkeley
|
| Street address |
327 Stanley Hall
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
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| Platforms (1) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA811518 |
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