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| Status |
Public on Jun 15, 2022 |
| Title |
Differential mRNA Expression Profiling Reveals the Role of MiR-375 in Inflammation of Bovine Mammary Epithelial Cells |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: to detect expression profile of differentially expressed mRNAs during bovine mammary epithelial cells (MAC-T) transfected with miR-375 inhibitor or negative control (NC) inhibitor in vitro. Methods: bovine mammary epithelial cells were transfected with miR-375 inhibitor or negative control (NC) inhibitor to assess the expression profiles of mRNAs using RNA-seq. Results: silencing miR-375 down-regulated and upregulated the expression of 48 and 15 mRNAs, respectively, in bovine mammary epithelial cells. Conclusion: miR-375 silencing dysregulated the expression of 63 mRNAs in bMECs. Also, miR-375 silencing increased the expression of NR4A1 and PTPN5 genes, all anti-inflammatory genes, via the MAPK signaling pathway. Given the negative correlation between miR-375 expression and NR4A1 and PTPN5 genes, miR-375 potentially promotes inflammation in the mammary gland through the MAPK signaling pathway. The findings of this study provide a new perspective of treating mastitis in cows.
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| Overall design |
Bovine mammary epithelial cells were seeded and cultured in 6-well plates. When the cells reached 60-70% confluence, they were transfected with miR-375 or negative control (NC) (final concentration 100nM). The experiment was performed in triplicate. Subsequently, all samples were used to perform the RNA-seq.
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| Contributor(s) |
Li Y, Hu Q, Luoreng Z, Yang J, Wang X, Ma Y, Wei D |
| Citation(s) |
35681895 |
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| Submission date |
Feb 26, 2022 |
| Last update date |
Jun 16, 2022 |
| Contact name |
Xing-Ping Wang |
| E-mail(s) |
wxp@nxu.edu.cn
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| Organization name |
Ningxia University
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| Department |
College of animal science and technology
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| Lab |
Key Laboratory of Ruminant Molecular Cell Breeding of Ningxia Hui Autonomous Region
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| Street address |
No. 489, Helan Shanxi Road, Xixia District
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| City |
Yinchuan |
| State/province |
Ningxia |
| ZIP/Postal code |
750021 |
| Country |
China |
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| Platforms (1) |
| GPL26012 |
Illumina NovaSeq 6000 (Bos taurus) |
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| Samples (6)
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| GSM5918595 |
Bovine mammary epithelial cells, NC inhibitor, rep1 |
| GSM5918596 |
Bovine mammary epithelial cells, NC inhibitor, rep2 |
| GSM5918597 |
Bovine mammary epithelial cells, NC inhibitor, rep3 |
| GSM5918598 |
Bovine mammary epithelial cells, miR-375 inhibitor, rep1 |
| GSM5918599 |
Bovine mammary epithelial cells, miR-375 inhibitor, rep2 |
| GSM5918600 |
Bovine mammary epithelial cells, miR-375 inhibitor, rep3 |
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| Relations |
| BioProject |
PRJNA810710 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE197498_RAW.tar |
820.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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