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| Status |
Public on Feb 22, 2023 |
| Title |
Meis1 establishes the pre-hemogenic endothelial state prior to Runx1 expression [CITE-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Hematopoietic stem and progenitor cells (HSPCs) originate from an endothelial-to-hematopoietic transition (EHT) during embryogenesis. Characterization of early hemogenic endothelial (HE) cells is required to understand what drives hemogenic specification and to accurately define cells capable of undergoing EHT. Using Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq), we defined the early subpopulation of pre-HE cells based on both surface markers and transcriptomes. We identified the transcription factor Meis1 as an essential regulator of hemogenic cell specification in the embryo prior to Runx1 expression. Meis1 is expressed at the earliest stages of EHT and distinguishes pre-HE cells primed towards the hemogenic trajectory from the arterial endothelial cells that continue towards a vascular fate. Endothelial-specific deletion of Meis1 impaired the formation of functional Runx1-expressing HE which significantly impeded the emergence of pre-HSPC via EHT. Our findings implicate Meis1 in a critical fate-determining step for establishing EHT potential in endothelial cells.
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| Overall design |
CITE-Seq profiles of CD31+ cells FACS-isolated from dissected mouse AGM at embryonic day 10.5. Each replicate represents a pool of 2-5 embryos.
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| Contributor(s) |
Coulombe P, Karsan A, Fentiman A, Parker JD, Tam D, Yung E, Humphries RK |
| Citation(s) |
37500618 |
| |
| Submission date |
Feb 22, 2022 |
| Last update date |
Jun 07, 2024 |
| Contact name |
Aly Karsan |
| E-mail(s) |
akarsan@bcgsc.ca
|
| Phone |
6047075900
|
| Organization name |
Canada's Michael Smith Genome Sciences Centre
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| Street address |
570 W 7th Ave, 100
|
| City |
Vancouver |
| State/province |
British Columbia |
| ZIP/Postal code |
V5Z 4S6 |
| Country |
Canada |
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| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL30172 |
NextSeq 2000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA809477 |
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