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| Status |
Public on Nov 14, 2022 |
| Title |
Expression data from regenerating human airway epithelium in vitro. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To identify transcriptomic signature of human airway epithelium as it undergoes full differentiation into mucociliated epithelial cells under air-liquid interface.
We used microarrays to detail the global gene expression pattern from day 0 through day 28 following air-liquid interface in human airway epithelial cells and identified distinct clusters of gene expression during this process.
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| Overall design |
In a time-course study, human small airway epithelial cells (hSAEpCs) were cultured in vitro and at successive points (days 0, 2, 4, 6, 8, 10, 14, 21 and 18 [labeled D0, D2, D4, and so forth] following air-liquid interface [ALI]) were lysed in situ, their RNA were extracted and hybridized on Affymetrix microarrays. Our objective here was to identify molecular signatures, as gene expression states for individual genes, that correlate with stage of hSAEpCs differentiation at various days post-ALI.
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| Contributor(s) |
Benam KH |
| Citation(s) |
36519134 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R41 ES031639 |
An Advanced Lung Organomimetic to Reproduce Human Airway Pathophysiology |
PNEUMAX, LLC |
Kambez Hajipouran Benam |
| U01 EB029085 |
A Microphysiological Mimicry of Human Lung-Bone Marrow Organ-Organ Crosstalk On-a-Chip |
UNIVERSITY OF PITTSBURGH AT PITTSBURGH |
Kambez Hajipouran Benam |
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| Submission date |
Feb 22, 2022 |
| Last update date |
Jan 05, 2023 |
| Contact name |
Kambez Hajipouran Benam |
| E-mail(s) |
BENAMK@pitt.edu
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| Organization name |
University of Pittsburgh
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| Street address |
200 Lothrop St, Rm E1144
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| City |
Pittsburgh |
| State/province |
Pennsylvania |
| ZIP/Postal code |
15213 |
| Country |
USA |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (27)
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| GSM5910075 |
hSAEpC at D0 post-ALI, biological replicate 1 |
| GSM5910076 |
hSAEpC at D0 post-ALI, biological replicate 2 |
| GSM5910077 |
hSAEpC at D0 post-ALI, biological replicate 3 |
| GSM5910078 |
hSAEpC at D2 post-ALI, biological replicate 1 |
| GSM5910079 |
hSAEpC at D2 post-ALI, biological replicate 2 |
| GSM5910080 |
hSAEpC at D2 post-ALI, biological replicate 3 |
| GSM5910081 |
hSAEpC at D4 post-ALI, biological replicate 1 |
| GSM5910082 |
hSAEpC at D4 post-ALI, biological replicate 2 |
| GSM5910083 |
hSAEpC at D4 post-ALI, biological replicate 3 |
| GSM5910084 |
hSAEpC at D6 post-ALI, biological replicate 1 |
| GSM5910085 |
hSAEpC at D6 post-ALI, biological replicate 2 |
| GSM5910086 |
hSAEpC at D6 post-ALI, biological replicate 3 |
| GSM5910087 |
hSAEpC at D8 post-ALI, biological replicate 1 |
| GSM5910088 |
hSAEpC at D8 post-ALI, biological replicate 2 |
| GSM5910089 |
hSAEpC at D8 post-ALI, biological replicate 3 |
| GSM5910090 |
hSAEpC at D10 post-ALI, biological replicate 1 |
| GSM5910091 |
hSAEpC at D10 post-ALI, biological replicate 2 |
| GSM5910092 |
hSAEpC at D10 post-ALI, biological replicate 3 |
| GSM5910093 |
hSAEpC at D14 post-ALI, biological replicate 1 |
| GSM5910094 |
hSAEpC at D14 post-ALI, biological replicate 2 |
| GSM5910095 |
hSAEpC at D14 post-ALI, biological replicate 3 |
| GSM5910096 |
hSAEpC at D21 post-ALI, biological replicate 1 |
| GSM5910097 |
hSAEpC at D21 post-ALI, biological replicate 2 |
| GSM5910098 |
hSAEpC at D21 post-ALI, biological replicate 3 |
| GSM5910099 |
hSAEpC at D28 post-ALI, biological replicate 1 |
| GSM5910100 |
hSAEpC at D28 post-ALI, biological replicate 2 |
| GSM5910101 |
hSAEpC at D28 post-ALI, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA809192 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE197142_RAW.tar |
216.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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