|
| Status |
Public on Jan 05, 2010 |
| Title |
Identification of LY6K target genes in bladder cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Five bladder cancer (BC) cell lines were subjected to high-resolution array comparative genomic hybridization (CGH), which contained about 244,000 probes. The focus was on the typical gain locus of 8q24.3, which has not been studied in detail for BC. Integrating array-CGH data with the results of gene-expression profiling using oligo-microarray analysis revealed that 34 genes on 8q24.3 were generally up-regulated in the cell lines. Among these 34 genes, lymphocyte antigen 6 complex locus K (LY6K), which is a cancer/testis antigen, was the most up-regulated one in the BC (13.76-fold) as well as in clinical BC samples (2.84-fold). Investigation molecular network of LY6K performed using another oligo-microarray of the LY6K transfectant demonstrated that 269 genes were generally up-regulated more than five-fold in the transfectant. Twenty-one percent of the up-regulated genes were annotated into ‘cell cycle’ category, suggesting that LY6K is a promising molecular target for BC.
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| |
|
| Overall design |
LY6K transfected cancer cells vs control cells
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| |
|
| Contributor(s) |
Seki N |
| Citation missing |
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| |
| Submission date |
Dec 31, 2009 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Naohiko Seki |
| E-mail(s) |
naoseki@faculty.chiba-u.jp
|
| Phone |
+81-43-226-2971
|
| Organization name |
Chiba University
|
| Department |
Functional Genomics
|
| Street address |
1-8-1, Inohana
|
| City |
Chiba |
| State/province |
Chiba |
| ZIP/Postal code |
260-8670 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
|
| Samples (1) |
| GSM492477 |
Gene expression analysis of LY6K transfectant |
|
| Relations |
| BioProject |
PRJNA121903 |
