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| Status |
Public on Nov 02, 2022 |
| Title |
MPA induced alterations in endometrial stromal cell transcriptome profile |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Normal endometrial stromal cells (ESCs) increase the inhibitory effect of progestin on EC cell proliferation via paracrine signaling, but the mechanisms involved remain unclear.ESCs had different morphological features between progestin-sensitive and -insensitive EC tissues. The purpose of this study was to detect differential gene expression in endometrial stromal cells after MPA treatment and to screen for potential secretory factors.
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| Overall design |
The NSC cells were digested and passaged routinely at logarithmic growth stage, and then inoculated in six-well plates at 1*105/well after adjusted cell concentration. After 24h of cell walling, the cells were observed to be 60%-70% confluent, and the phenol red-free medium was starved for 8h, then replaced with phenol red-free medium supplemented with 2% charcoal-stripped FBS, and treated with ethanol or 10μM MPA for 6h, and then Trizol method was used to extract RNA. RNA-Seq was performed by Genergy Bio-Technology (Shanghai, China).
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| Contributor(s) |
Chen X, Cheng Y |
| Citation(s) |
35388173 |
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| Submission date |
Feb 16, 2022 |
| Last update date |
Nov 02, 2022 |
| Contact name |
Xiaojun Chen |
| E-mail(s) |
Xiaojunchen2013@sina.com
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| Organization name |
Obstetrics and Gynecology Hospital of Fudan University
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| Street address |
No. 419, Fangxie Road
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| City |
Shanghai |
| ZIP/Postal code |
200011 |
| Country |
China |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA807673 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE196890_gene_exp_with_symbol_R.txt.gz |
441.5 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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