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| Status |
Public on May 30, 2022 |
| Title |
Mapping cis-regulatory elements in human excitatory and inhibitory neurons links psychiatric disease heritability and activity-regulated transcriptional programs [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Genome-wide association studies (GWAS) have identified hundreds of loci associated with psychiatric diseases, yet there is a lack of understanding of disease pathophysiology. Common risk variants can shed light on the molecular mechanisms underlying these diseases; however, identifying specific causal variants remains challenging. An added complication is that most common risk variants are in non-coding regions. Their impact is likely to be restricted to particular cell types, developmental stages, or cell states. Here, we comprehensively mapped cis-regulatory elements in two defined populations of human excitatory and inhibitory neurons derived from pluripotent stem cells.
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| Overall design |
We performed bulk RNA-seq to measure transcriptomic profiles in human induced glutamatergic (Glu) and GABAergic (GABA) neurons derived from human pluripotent stem cells (ES and iPSCs). We exposed neurons to an elevated level of potassium chloride (KCl) to study activity-induced responses, and profiled gene expression at various timepoints after depolarization.
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| Contributor(s) |
Sanchez-Priego C, Hu R, Boshans L, Lalli M, Yang N |
| Citation(s) |
35649373 |
| |
| Submission date |
Feb 16, 2022 |
| Last update date |
Jun 02, 2022 |
| Contact name |
Nan Yan |
| E-mail(s) |
linda.lee2@mssm.edu, nan.yang1@mssm.edu
|
| Organization name |
Mount Sinai
|
| Department |
Neuroscience
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| Lab |
Yang Lab
|
| Street address |
1425 Madison Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (30)
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| This SubSeries is part of SuperSeries: |
| GSE196856 |
Mapping cis-regulatory elements in human excitatory and inhibitory neurons links psychiatric disease heritability and activity-regulated transcriptional programs |
|
| Relations |
| BioProject |
PRJNA807598 |
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