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Series GSE196699 Query DataSets for GSE196699
Status Public on Jun 06, 2022
Title RNA-seq analysis in adipocytes that lack APPBP2 and/or PRDM16
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We systematically determined the extent to which APPBP2 loss stimulate beige adipocyte biogenesis in a PRDM16-dependent fashion. To this end, we ran RNA-seq analysis in adipocytes that lack APPBP2 and/or PRDM16. By overlapping the transcriptome data with published dataset of brown/beige fat (Chondronikola et al., 2016; Perdikari et al., 2018; Shinoda et al., 2015a), we found that APPBP2 loss significantly increased 72 genes whose expression was enriched in brown adipocytes relative to white fat. Among 72 brown/beige fat-enriched genes, we found that 90.28% (65/72 genes) of them were regulated in a PRDM16-dependent manner. The data suggest that PRDM16 is required for the majority, if not all, of APPBP2’s action on brown fat genes
 
Overall design mRNA profiles of vehicle or cAMP treated inguinal adipocytes that lack APPBP2 and/or PRDM16
 
Contributor(s) Kajimura S, Wang Q
Citation(s) 35978186
Submission date Feb 14, 2022
Last update date Sep 05, 2022
Contact name NORC Boston
E-mail(s) fungen.core@gmail.com
Organization name Beth Israel Deaconess Medical Center
Street address 3 Blackfan Circle, CLS0733
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (24)
GSM5898930 Prdm16-KO+shAppbp2+cAMP inguinal white adipoctyes mRNA rep. 1
GSM5898931 Prdm16-KO+shAppbp2+cAMP inguinal white adipoctyes mRNA rep. 2
GSM5898932 Prdm16-KO+shAppbp2+cAMP inguinal white adipoctyes mRNA rep. 3
Relations
BioProject PRJNA806908

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Supplementary file Size Download File type/resource
GSE196699_RAW.tar 38.7 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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