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| Status |
Public on Jun 03, 2022 |
| Title |
The Old Yellow Enzyme OfrA fosters Staphylococcus aureus survival via thiol-dependent redox homeostasis |
| Organism |
Staphylococcus aureus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Old yellow enzymes (OYEs) are widely found in the bacterial, fungal, and plant kingdoms but absent in humans and have been used as biocatalysts for decades. However, OYEs physiological function in bacterial stress response and infection situations remained enigmatic. In the mode of pathogen, the gram-positive bacterium Staphylococcus aureus adapts to numerous stress conditions during pathogenesis. Here we show that in S. aureus genome, two paralogous genes (ofrA and ofrB) encode for two OYEs. We conducted bioinformatic analysis and found that ofrA is conserved among all publicly available representative staphylococcal genomes and some Firmicutes. Expression of ofrA is induced by electrophilic, oxidative, and hypochlorite stress in S. aureus. Furthermore, ofrA contributes to S. aureus survival against reactive electrophilic, oxygen, and chlorine species (RES, ROS, RCS) via thiol-dependent redox homeostasis. At the host-pathogen interface, ofrA mutation affects S. aureus survival in macrophages and whole human blood and the virulence factor staphyloxanthin production. Overall, our results shed the light onto a novel stress response strategy in the bacterial kingdom especially in the important human pathogen S. aureus.
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| Overall design |
3 replicates of S. aureus JE2 and 3 replicates of JE2ΔofrA in RPMI-1640 medium
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| Contributor(s) |
Ibrahim ES, Ohlsen K |
| Citation(s) |
35656003 |
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| Submission date |
Feb 14, 2022 |
| Last update date |
Jun 07, 2022 |
| Contact name |
Eslam S. Ibrahim |
| E-mail(s) |
eslam.ebrahim@pharma.cu.edu.eg, eslam_samir_ragab.ibrahim@uni-wuerzburg.de
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| Organization name |
Institut für Molekulare Infektionsbiologie (IMIB)
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| Street address |
Josef-Schneider-Straße 2, Bau D15
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| City |
Würzburg |
| ZIP/Postal code |
97080 |
| Country |
Germany |
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| Platforms (1) |
| GPL24034 |
Illumina NextSeq 500 (Staphylococcus aureus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA806843 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE196683_RAW.tar |
91.5 Mb |
(http)(custom) |
TAR (of WIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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