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| Status |
Public on Oct 14, 2022 |
| Title |
Analysis of the liver transcriptome from wild-type and Gpr151 knock-out mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The goal of this study is to identify genes and molecular pathways whose expression is altered in the livers of Gpr151 knock-out (KO) mice compared to Gpr151 wild-type (WT).
Methods: Total RNA was isolated from livers of fasted (5h) 16-week-old male mice. Deep sequencing of RNA from three wild-type and three knock-out mice was done using the mRNA-Seq pipeline at Novogene. The sequence reads that passed quality filters were aligned to the mouse GRCm38.p6 genome using STAR 2.6.1d. Differential expression testing was conducted using DESeq2.
Results: Using an optimized data analysis workflow, we mapped about 40 million sequence reads per sample to the mouse genome (build mm10) and identified 54,532 transcripts in the livers of Gpr151 WT and KO mice. RNA-seq data identified 79 transcripts which were significantly upregulated in KO (p-adj < 0.05, log2FoldChange>1) and 338 transcripts which were significantly downregulated (p-adj<0.05, log2FoldChange<-1) in KO.
Conclusions: Our study represents the first detailed analysis of the liver transcriptome of Gpr151 KO mice.
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| Overall design |
Hepatic mRNA profiles of 16-week-old Gpr151 WT and KO mice which were fed high-fast diet from 4 weeks of age.
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| Contributor(s) |
Bielczyk-Maczynska E, Knowles JW |
| Citation(s) |
36456565 |
| |
| Submission date |
Feb 10, 2022 |
| Last update date |
Dec 16, 2022 |
| Contact name |
Ewa Bielczyk-Maczynska |
| E-mail(s) |
ewabm@stanford.edu
|
| Phone |
6506952773
|
| Organization name |
Stanford University
|
| Department |
Division of Cardiovascular Medicine
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| Lab |
Knowles Lab
|
| Street address |
Falk Cardiovascular Research Center, 870 Quarry Rd
|
| City |
Palo Alto |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA805274 |
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