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| Status |
Public on Mar 25, 2010 |
| Title |
SEA BREAM PATH_INTEST |
| Platform organism |
Sparus aurata |
| Sample organisms |
Sparus aurata; Enteromyxum leei |
| Experiment type |
Expression profiling by array
|
| Summary |
To determine the effects on the intestine gene expression of pathogen exposure to Enteromyxum leei. One fish group was exposed to E. leei-contaminated effluent (recipient group = R) . R fish (n= 66, average weight = 134 g) were placed in two replicated 200L fibre-glass tanks which were set to receive exclusively the effluent water from another tank containing 24 infected (donors = D; average weight = 127.3 g; prevalence of infection = 54%) gilthead sea bream. The D to R fish ratio was 0.8. Other 66 naïve fish were allocated in two replicated tanks (control group = CTRL) under the same conditions, but without receiving contaminated effluent. Over the course of the study, day length followed natural changes and water was heated in order to keep temperature always above 18ºC, the range was 18-23 ºC. Water was 5 µm-filtered and UV irradiated, and salinity was 37.5‰. Water flow was 10L/min and oxygen content of outlet water remained higher than 85%saturation. All fish were fed daily a commercial dry pellet diet at about 1% of body weight. Disease signs and daily mortalities were recorded throughout the experiments. The parasitic status of dead fish was checked by microscopic examination of fresh intestinal scrapings. Fish were sampled after 113 days post exposure (p.e.). Feeding was stopped one day prior to the sampling to ensure that the digestive tract was empty. Head kidney and posterior intestine were rapidly excised, frozen in liquid nitrogen, and stored at -80 °C until RNA extraction and analysis.
Keywords: Treated/Untreated, confinement, cortisol, stress response, time course, microarray
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| Overall design |
28 intestine samples - Twenty eight slides were hybridised using a reference design. Three groups (control, infected and non-infected) were compared using five individual fish in each group. Each sample was hybridised twice - the second being a dye-swap of the first.
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| Web link |
http://www.sigenae.org/aquafirst/
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| Contributor(s) |
Cairns MT, Davey G, Calduch-Giner J, Pérez-Sánchez J, Houeix B, Talbot A, Reinhardt R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 24, 2009 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Michael Taylor Cairns |
| E-mail(s) |
michael.cairns@nuigalway.ie
|
| Phone |
0035391492094
|
| Organization name |
NUI Galway
|
| Department |
MRI
|
| Lab |
Rm 104
|
| Street address |
University Road
|
| City |
Galway |
| ZIP/Postal code |
n/a |
| Country |
Ireland |
| |
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| Platforms (1) |
| GPL10126 |
AQUAFIRST_seabream_18K_Ver1 (condensed) |
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| Samples (28)
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| This SubSeries is part of SuperSeries: |
| GSE20619 |
Gilthead seabream pathogen exposure to Enteromyxum leei |
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| Relations |
| BioProject |
PRJNA129739 |
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