|
| Status |
Public on Sep 01, 2022 |
| Title |
Single cell RNA and TCR profiling of tetramer sorted mouse mLN and IELs. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
We utilized H2-I-Ab tetramers to isolate CD4 T cells from mice to characterize the functional characteristics of ileal intraepithelial T cells and their lymphoid counterparts in the corresponding draining lymph nodes (mLN). Non-CD4 T cells (B220+, CD11b+, CD11c+, F4/80+, CD8a+) were excluded before FACS-sorting of CD45+CD3+CD4+ tetramer-specific T cells discriminated by double-positive staining with dual-labeled tetramer fluorophores. We recovered tetramer+ and tetramer- IELs and mLN T cells, and profiled them by single-cell RNA and TCR sequencing.
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| |
|
| Overall design |
FACS sort of tetramer positive and tetramer negative cells in mLN and IELs.
|
| |
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| Contributor(s) |
Plichta DR, Brown E, Graham DB, Pedersen TK |
| Citation(s) |
36115338 |
| |
| Submission date |
Feb 09, 2022 |
| Last update date |
Nov 01, 2022 |
| Contact name |
Damian Plichta |
| E-mail(s) |
damian@broadinstitute.org
|
| Organization name |
Broad Institute
|
| Street address |
415 Main Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
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| Platforms (2) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA804878 |
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