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| Status |
Public on May 23, 2011 |
| Title |
Expression data from E10.5 mouse otocyst |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We established a novel EGFP reporter mouse line (named Tg(ETAR-EGFP)14Imeg), which enables the placode-derived inner ear sensory cell lineage to be visualized and monitored. At E10.5, EGFP expression was detected in the ventral and dorsomedial region of the otocyst.
Using this reporter line and FACS-array technology, we performed gene expression profiling focused on the regional specificity of the otocyst.
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| Overall design |
We sorted E10.5 otocyst epithelial cells of heterozygous mice into EGFP-positive and EGFP-negative cells for RNA extraction and hybridization on Affymetrix microarrays. We profiled the expression of the genes in FACS-enriched cell population and conducted transcriptome analysis.
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| Contributor(s) |
Fujimoto C, Aburatani H |
| Citation(s) |
20963824 |
| |
| Submission date |
Dec 23, 2009 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Chisato Fujimoto |
| Organization name |
The University of Tokyo
|
| Street address |
7-3-1 Hongo
|
| City |
Bunkyo-ku Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA122569 |
