|
| Status |
Public on Feb 20, 2022 |
| Title |
Genome-wide mapping of H3K27 tri-methylation in PRC2 components of Pyricularia oryzae |
| Organism |
Pyricularia oryzae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report different roles of three PRC2 components, KMT6, P55 and SIN3 played in distribution of genome-wide H3K27 tri-methylation of Pyricularia oryzae. Polycomb repressive complex 2 (PRC2), which is composed of Ezh(Kmt6), Su(z)12, Esc(Eed) and accessory subunits RbAp48/Nurf55(P55), catalyzes the methylation of histone H3 at lysine 27. Loss of P55 caused severe global defects in the normal distribution of H3K27me3 and transcriptional reprogramming on the H3K27me3-occupied genes. Furthermore, we found that the Sin3 histone deacetylase complex was required to sustain H3K27me3 occupancy and stably maintain gene repression by directly interacting with P55.
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| |
|
| Overall design |
Examination of H3K27 tri-methylation of different PRC2 components of Pyricularia oryzae. Two biological replicates were performed.
|
| Web link |
https://doi.org/10.1111/nph.18383
|
| |
|
| Contributor(s) |
Tao Z |
| Citation(s) |
35842786 |
| |
| Submission date |
Feb 01, 2022 |
| Last update date |
Aug 08, 2022 |
| Contact name |
Chuyu Lin |
| E-mail(s) |
12316066@zju.edu.cn
|
| Phone |
+86 13588301175
|
| Organization name |
Zhejiang University
|
| Department |
College of Agriculture and Biotechnology
|
| Lab |
Tao’s Lab
|
| Street address |
866#, Yuhangtang Road
|
| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310058 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL29717 |
HiSeq X Ten (Pyricularia oryzae) |
|
| Samples (16)
|
| GSM5852329 |
P. oryzae mycelia - H3K27me3_WT_rep1_input |
| GSM5852330 |
P. oryzae mycelia - H3K27me3_WT_rep1_IP |
| GSM5852331 |
P. oryzae mycelia - H3K27me3_WT_rep2_input |
| GSM5852332 |
P. oryzae mycelia - H3K27me3_WT_rep2_IP |
| GSM5852333 |
P. oryzae mycelia - H3K27me3_kmt6_rep1_input |
| GSM5852334 |
P. oryzae mycelia - H3K27me3_kmt6_rep1_IP |
| GSM5852335 |
P. oryzae mycelia - H3K27me3_kmt6_rep2_input |
| GSM5852336 |
P. oryzae mycelia - H3K27me3_kmt6_rep2_IP |
| GSM5852337 |
P. oryzae mycelia - H3K27me3_p55_rep1_input |
| GSM5852338 |
P. oryzae mycelia - H3K27me3_p55_rep1_IP |
| GSM5852339 |
P. oryzae mycelia - H3K27me3_p55_rep2_input |
| GSM5852340 |
P. oryzae mycelia - H3K27me3_p55_rep2_IP |
| GSM5852341 |
P. oryzae mycelia - H3K27me3_sin3_rep1_input |
| GSM5852342 |
P. oryzae mycelia - H3K27me3_sin3_rep1_IP |
| GSM5852343 |
P. oryzae mycelia - H3K27me3_sin3_rep2_input |
| GSM5852344 |
P. oryzae mycelia - H3K27me3_sin3_rep2_IP |
|
| Relations |
| BioProject |
PRJNA802372 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE195863_RAW.tar |
1.8 Gb |
(http)(custom) |
TAR (of BEDGRAPH) |
| GSE195863_WT_vs_input_DE_Peaks_f_2.txt.gz |
88.9 Kb |
(ftp)(http) |
TXT |
| GSE195863_kmt6_vs_background_H3K27me3_DE_Peaks.txt.gz |
85.6 Kb |
(ftp)(http) |
TXT |
| GSE195863_kmt6_vs_input_DE_Peaks_f_2.txt.gz |
2.8 Kb |
(ftp)(http) |
TXT |
| GSE195863_p55_vs_background_H3K27me3_DE_Peaks.txt.gz |
35.1 Kb |
(ftp)(http) |
TXT |
| GSE195863_p55_vs_input_DE_Peaks_f_2.txt.gz |
41.1 Kb |
(ftp)(http) |
TXT |
| GSE195863_sin3_vs_background_H3K27me3_DE_Peaks.txt.gz |
73.1 Kb |
(ftp)(http) |
TXT |
| GSE195863_sin3_vs_input_DE_Peaks_f_2.txt.gz |
74.2 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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