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| Status |
Public on May 04, 2024 |
| Title |
Noncoding RNA HID1 promotes phyB-dependent seed germination by repressing the biosynthesis of ABA [RNA-seq] |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Arabidopsis noncoding RNA HID1 acts as a positive regulator to promote phyB-mediated seed germination by modulating the biosynthesis of abscisic acid.
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| Overall design |
Three biological replicates of each genotype were used for high-throughput sequencing analysis. For seed germination-related experiments, all plants were grown side-by-side, and the seeds were harvested on the same day. The seeds were dried at room temperature for about 2 months prior to subsequent experiments. Including sterilization and plating, seeds were exposed to white light for 1 hour. For the phyB-off condition, the seeds were exposed to far-red light (5 μmol·m-2·s-1) for 5 min to inactivate phyB. For the phyB-on condition, the seeds were irradiated with red light (20 μmol·m-2·s-1) for 5 min to activate phyB. The seeds were then incubated in the dark for the indicated time, and the germination rates were determined in the dark room.
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| Web link |
https://academic.oup.com/plcell/article/35/2/700/6845762
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| Contributor(s) |
Wang Y, Fan Y, Fan D, Deng XW, Zhu D |
| Citation(s) |
36423345 |
| Submission date |
Jan 30, 2022 |
| Last update date |
May 04, 2024 |
| Contact name |
Yangyang Fan |
| E-mail(s) |
fanyangyang123456@126.com
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| Organization name |
Peking University
|
| Street address |
No.5 Yiheyuan Road Haidian District,
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| City |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (1) |
| GPL17639 |
Illumina HiSeq 2500 (Arabidopsis thaliana) |
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| Samples (27)
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| This SubSeries is part of SuperSeries: |
| GSE195748 |
Noncoding RNA HID1 promotes phyB-dependent seed germination by repressing the biosynthesis of ABA. |
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| Relations |
| BioProject |
PRJNA802053 |
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