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| Status |
Public on Jan 04, 2012 |
| Title |
Gene expression changes in the human diaphragm following cardiothoracic surgery |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
It is unknown how soon the diaphragm begins to start the process of atrophy following the start of MV. We hypothesized that genes responsible for maintaining diaphragmatic contractile function, stress response, energy transduction would be altered over the course of a 5 hour cardiothoracic surgery.
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| Overall design |
Two diaphragm biopsies were obtained from 5 male patients (~67 +/- 11 years) undergoing cardiothoracic surgery. The first biopsy was obtained as soon as the diaphragm was exposed; the second biopsy was obtained as late in the surgery as possible (4.9 +/- 1.8 hours). We profiled mRNA from the 5 pairs of muscle biopsies with a microarray (Affymetrix Human Genome U133 Plus 2.0).
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| Contributor(s) |
Huang T, Deoghare HV, Smith BK, Beaver TM, Baker HV, Martin AD |
| Citation(s) |
21463877 |
| |
| Submission date |
Dec 17, 2009 |
| Last update date |
Mar 25, 2019 |
| Contact name |
M Cecilia Lopez |
| E-mail(s) |
mclopez@ufl.edu
|
| Phone |
352 273-8133
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| Organization name |
University of Florida
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| Department |
Molecular Genetics and Microbiology
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| Lab |
Microarray lab 103
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| Street address |
1376 Mowry Road
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610-3610 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA122253 |
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