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| Status |
Public on Sep 30, 2024 |
| Title |
Genome-wide transcriptome analysis of human cell models exposed to Borrelia burgdorferi |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We used high-throughput sequencing to investigate the genome-wide transcriptional response in human cells to treatment with Borrelia burgdorferi. We chose a time point of 72 h as ticks feed on their host for several days and at the same time the early response in Lyme disease is expected to occur at this time period at a cellular level. We found that the two cell models studied (HUVEC and HEK-293 cells) had significantly different responses. More significantly differentially expressed genes (69 in total) were found in HUVEC cells than in HEK-293 cells (8 in total). Functional analysis indicated induction of the immune response in HUVEC and suggest changes in the extracellular matrix in HEK-293.
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| Overall design |
Examination of gene expression with RNA-seq in HUVEC and HEK-293 cells in response to Borrelia burgdorferi.
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| Contributor(s) |
Berthold A, Lloyd V, Wajnberg G |
| Citation(s) |
39202370 |
| |
| Submission date |
Jan 24, 2022 |
| Last update date |
Sep 30, 2024 |
| Contact name |
Anne Berthold |
| Organization name |
Mount Allison University
|
| Department |
Biology
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| Lab |
Lloyd tick lab
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| Street address |
62 York Street
|
| City |
Sackville |
| State/province |
New Brunswick |
| ZIP/Postal code |
E4L 1E2 |
| Country |
Canada |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA800079 |
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