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| Status |
Public on Aug 14, 2023 |
| Title |
Single-cell analysis of human MAIT cell transcriptional, functional and clonal diversity [Exp 1] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
MAIT cells are innate-like T cells that recognize microbial metabolites through a semi-invariant T cell receptor (TCR). Major questions remain regarding the extent of human MAIT cell functional and clonal diversity. To address these, we analyzed the single-cell transcriptome and TCR repertoire of blood and liver MAIT cells and developed functional RNA-sequencing (fRNA-seq), a method to integrate function and TCR clonotype at single-cell resolution. MAIT cell clonal diversity was comparable to conventional memory T cells, with private TCR repertoires shared across matched tissues. Baseline functional diversity was low and largely related to tissue site. MAIT cells showed stimulus-specific transcriptional responses in vitro, with cells positioned along gradients of activation. Clonal identity influenced resting and activated transcriptional profiles, but intriguingly was not associated with the capacity to produce IL-17. Overall, MAIT cells show phenotypic and functional diversity according to tissue localization, stimulation environment, and clonotype.
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| Overall design |
MAIT cells (CD3+MR1/5-OP-RU+) were sorted from matched human blood (n = 3) and liver (n = 4) for single-cell RNA-sequencing (scRNA-seq) and single-cell TCR-sequencing (scTCR-seq) using 10x Genomics technology.
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| Web link |
https://www.nature.com/articles/s41590-023-01575-1
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| |
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| Contributor(s) |
Garner LC, Provine NM, Klenerman P |
| Citation(s) |
37580605 |
| |
| Submission date |
Jan 21, 2022 |
| Last update date |
Aug 28, 2023 |
| Contact name |
Lucy Garner |
| E-mail(s) |
lucy.garner@ndm.ox.ac.uk
|
| Organization name |
University of Oxford
|
| Department |
Nuffield Department of Medicine
|
| Street address |
Experimental Medicine, Level 5, John Radcliffe Hospital
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| City |
Oxford |
| ZIP/Postal code |
OX3 9DU |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (22)
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| This SubSeries is part of SuperSeries: |
| GSE194189 |
Single-cell analysis of human MAIT cell transcriptional, functional and clonal diversity |
|
| Relations |
| BioProject |
PRJNA799380 |
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