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| Status |
Public on Apr 13, 2022 |
| Title |
Inhibitory mechanism of combined hydroxychavicol with epigallocatechin-3-gallate against glioma cancer cell lines: A transcriptomic analysis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We reported the molecular mechanisms of low combination doses of epigallocatechin-3-gallate and hydroxychavicol (EGCG+HC) in glioma cell lines 1321N1 and LN18. Using high throughput RNA sequencing, combined EGCG+HC exerted its anticancer effect by downregulating the axon guidance process and metabolic pathways, while simultaneously interfering with endoplasmic reticulum unfolded protein response pathway. Furthermore, EGCG+HC exerted its apoptotic effect through the alteration of mitochondrial genes such as MT-CO3 and MT-RNR2 in 1321N1 and LN18 cells respectively. EGCG+HC dynamically altered DYNLL1 alternative splicing expression in 1321N1 and DLD splicing expression in LN18 cell lines.
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| Overall design |
Transcriptomic profiles of glioma cell lines (1321N1, LN18) treated with EGCG+HC, EGCG, HC and vehicle control
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| Contributor(s) |
Abdul Rahman A, Wan Ngah WZ, Jamal R, Makpol S, Harun R, Mokhtar N |
| Citation(s) |
35392560 |
| |
| Submission date |
Jan 17, 2022 |
| Last update date |
Apr 13, 2022 |
| Contact name |
Amirah Abdul Rahman |
| E-mail(s) |
amirahar@uitm.edu.my
|
| Organization name |
Universiti Teknologi MARA
|
| Department |
Dept. of Biochemistry & Molecular Medicine
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| Lab |
Faculty of Medicine
|
| Street address |
Sungai Buloh Campus, UiTM Cawangan Selangor
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| City |
Sungai Buloh |
| State/province |
SELANGOR |
| ZIP/Postal code |
47000 |
| Country |
Malaysia |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA798066 |
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