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| Status |
Public on Mar 31, 2022 |
| Title |
Estrogen Modulation of the Pronociceptive Effects of Serotonin on Female Rat Trigeminal Sensory Neurons is Timing- and Dosage-Dependent and Requires Estrogen Receptor Alpha |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Since estrogen is a major hormone involved in multiple gene expression pathways in the cell, we wanted to determine the corresponding changes in trigeminal ganglia transcriptome of the E2-treated rats. Adult ovariectomized female rats were subcutaneneously implanted with either 5% (physiological level comparable to diestrus; 15-20 pg/ml; one capsule), 20% (physiological level comparable to proestrus; 40-60 pg/ml; 2 capsules of 10%), 40% (supraphysiological level; 2 capsules of 20%) E2, or control (2 capsules of 100% cholesterol) capsules. One week after implantation, nocifensive behaviors were recorded and trigeminal ganglia tissue was extracted.
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| Overall design |
Total RNA was extracted from the right trigeminal ganglia (TG) tissue (n=3 per hormone treatment group) and sequenced [Novogene; sequencing lab at The University of California at Davis campus]
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| Contributor(s) |
Kaur S, Hickman TM, Lopez-Ramirez A, McDonald H, Lockhart LM, Darwish O, Averitt DL |
| Citation(s) |
35121697 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R15 DE025970 |
Sex Differences in Serotonergic Modulation of Trigeminal Pain |
TEXAS WOMAN'S UNIVERSITY |
Dayna Loyd Averitt |
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| Submission date |
Jan 16, 2022 |
| Last update date |
Apr 02, 2022 |
| Contact name |
Dayna Loyd Averitt |
| E-mail(s) |
daveritt@twu.edu
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| Phone |
9408982708
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| Organization name |
Texas Woman's University
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| Department |
Biology
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| Street address |
1000 Old Main Circle
|
| City |
Denton |
| State/province |
Texas |
| ZIP/Postal code |
76204 |
| Country |
USA |
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| Platforms (1) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA797795 |
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