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| Status |
Public on Mar 13, 2023 |
| Title |
Single cell transcriptomic and VDJ sequencing of antibody-secreting cells (ASCs) from the bone marrow, spleen and thymus of young (3 months old) female and male mice. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: To examine the trasncriptional and VDJ repertoire differences that exist in ASCs based on factors such as tissue localiation and sex.
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| Overall design |
ASCs were flow cytometrically purified from the bone marrow, spleen and thymus of young (3 months old) female and male Prdm1-eYFP mice. For each sort, pools of 3 mice were used. ASCs were sorted as B220+/- CD138HI IgD- CD90.2- Prdm1-eYFP+. Single cell gene expression and VDJ libraries were constructed via 10x Genomics technology.
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| |
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| Contributor(s) |
Pioli PD |
| Citation(s) |
36890795 |
| |
| Submission date |
Jan 14, 2022 |
| Last update date |
Mar 14, 2023 |
| Contact name |
Peter Dion Pioli |
| E-mail(s) |
peter.pioli@usask.ca
|
| Phone |
1-639-994-8478
|
| Organization name |
University of Saskatchewan
|
| Department |
Biochemistry, Microbiology and Immunology
|
| Street address |
107 Wiggins Rd
|
| City |
Saskatoon |
| State/province |
SK |
| ZIP/Postal code |
S7N 5E5 |
| Country |
Canada |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA797279 |
| SRA |
SRP355069 |
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