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Series GSE19360 Query DataSets for GSE19360
Status Public on Jul 14, 2010
Title Integrated array-CGH and expression microarray analyses on medulloblastomas in heterozygous Ptch1 mice, expression
Organism Mus musculus
Experiment type Expression profiling by array
Summary Genomic radiation signature illuminates low-dose effects with sharply reflected transcriptome in Ptch1-deficient medulloblastomas.
Cancer risks of low-dose radiation are of great concern especially in relation to rapidly increasing medical exposures; however, their accurate assessments cope with many challenges and difficulties, partly due to the inability to distinguish radiation-induced tumors from spontaneous ones. Here, we analyzed the dose-dependent effect of radiation on medulloblastoma development in Ptch1 heterozygous mice on C3B6F1 background. The incidence and latency of medulloblastoma increased and shortened with increasing radiation dose, respectively. Amazingly, radiation contributed to tumorigenesis even at 50 mGy and 100% of mice got medulloblastoma with 1.5 Gy. Loss of heterozygosity (LOH) analysis on a total of 164 tumors indicated that spontaneous tumors showed LOH in broad regions on chromosome 13, including Ptch1 and distally-extending telomeric portion (S-type). In contrast, tumors developed after 3 Gy irradiation exhibited interstitial losses around Ptch1 (R-type). A clear dose-dependent increase in the proportion of R-type tumor at intermediate doses suggested R-type to be a reliable radiation signature. Array-CGH analysis indicated the R-type-specific copy-number reduction around Ptch1 and LOH-type-independent frequent gains of whole chromosome 6. Integrated expression microarray analysis indicated that expression levels of many genes within the altered genomic regions faithfully reflected the genomic copy-number changes. Furthermore, it was also suggested that these expression changes in turn influenced on many other genes, such as Tgfb2 and Tgfb3, on widespread genomic regions. This is the first demonstration that radiation-induced tumors developed after low-dose irradiation can be characterized quite precisely by interstitial deletion of Ptch1 and by associated gene expression profile.
Overall design Gene expression in 3 normal cerebellum tissues and 12 medulloblastomas was measured.
Contributor(s) Ishida Y, Takabatake T, Kakinuma S, Doi K, Yamauchi K, Kaminishi M, Kito S, Ohta Y, Amasaki T, Moritake H, Kokubo T, Nishimura M, Nishikawa T, Hino O, Shimada Y
Citation(s) 20616149
Submission date Dec 07, 2009
Last update date Jan 12, 2017
Contact name Takashi Takabatake
Organization name National Institute of Radiological Sciences
Street address Anagawa 4-9-1, Inage-ku
City Chiba
State/province Chiba
ZIP/Postal code 263-8555
Country Japan
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (15)
GSM480629 Normal cerebellum 1 (S15407)
GSM480630 Normal cerebellum 2 (S15409)
GSM480631 Normal cerebellum 3 (S15560)
This SubSeries is part of SuperSeries:
GSE19384 Integrated array-CGH and expression microarray analyses on medulloblastomas in heterozygous Ptch1 mice
BioProject PRJNA123895

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19360_RAW.tar 28.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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