|
| Status |
Public on Jan 14, 2022 |
| Title |
ERK-Mediated Phosphorylation of OLA1 Controls Adaptive Mitochondria to Nucleus Communication Regulating Cellular Homeostasis [ChIP-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We found that changes in mitochondrial membrane potential or ROS generation trigger the translocation of OLA1 from mitochondria and cytoplasm to the nucleus, regulated by ERK1-mediated phosphorylation of OLA1 on Ser232/Tyr236. Subsequent phosphorylation of nuclear-OLA1 on Thre325 by ERK2 activates its function as a genetic factor regulating cellular homeostasis by modulating HDAC9 expression. Disruption of OLA1 phosphorylation blocks its nuclear translocation, compromised the expression of nuclear-encoded mitochondrial proteins, DNA damage response, and multiple stress-response-related-genes, leading to cellular dysfunction. Our findings reveal that OLA1 is a crucial component of mitonuclear response regulatory hubs essential for regulating cellular adaptation to stress.
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| |
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| Overall design |
Examination of the role of OLA1 as a transcription factor.
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| |
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| Contributor(s) |
Afolayan AJ, Sidlowski P |
| Citation missing |
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| |
| Submission date |
Jan 09, 2022 |
| Last update date |
Jan 16, 2022 |
| Contact name |
Adeleye J. Afolayan |
| E-mail(s) |
aafolayan@mcw.edu
|
| Phone |
4149555634
|
| Organization name |
Medical College of Wisconsin
|
| Street address |
8701 Watertown Plank Rd., PD278, CRI TBRC, Rm C3278
|
| City |
Milwaukee |
| State/province |
Wisconsin |
| ZIP/Postal code |
53226 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (4)
|
| GSM5792021 |
Pulmonary endothelial cells - ECCTRL_1 [ChIP-seq] |
| GSM5792022 |
Pulmonary endothelial cells - ECCTRL_2 [ChIP-seq] |
| GSM5792023 |
Pulmonary endothelial cells - ECCTRL_3 [ChIP-seq] |
| GSM5792024 |
Pulmonary endothelial cells - ECOLA_NLS_1 [ChIP-seq] |
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| This SubSeries is part of SuperSeries: |
| GSE193319 |
ERK-Mediated Phosphorylation of OLA1 Controls Adaptive Mitochondria to Nucleus Communication Regulating Cellular Homeostasis |
|
| Relations |
| BioProject |
PRJNA795788 |
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