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| Status |
Public on Mar 09, 2022 |
| Title |
The dynamic change of immune responses between acute and recrudescence stages of rodent malaria infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Malaria infections are persistent as frequent recrudescence of the disease may occur following the acute infection stage, but the different immune responses that control the acute and recrudescence stages are still largely unknown. Using single-cell RNA sequencing (scRNA-seq), we showed that the number of Th1 and plasma cells in the spleen was significantly reduced during the recurrence stage compared to the acute stage of Plasmodium chabaudi chabaudi AS (P. chabaudi) infection. Additionally, the ability of both CD4+ T cell responses and B cells to control P. chabaudi recurrence was significantly reduced compared to their roles in the control of acute infection. In contrast, the number of innate immune cells, including red pulp macrophages (RPMs), gamma delta (γδ) T cells, Dendritic cells (DCs), and neutrophils were significantly increased during the recurrence stage and demonstrated to be critical for P. chabaudi infection recurrence control. Thus, our data strongly suggest the complementary role of innate immune responses in controlling malaria recrudescence when adaptive immune responses are suppressed. These findings shed new light on the development of immune interventions against malaria.
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| Overall design |
sc-RNASeq analysis of splenocytes isolated from P. chabaudi-infected mice at Day0, Day8 and Day16 post-infection.
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| Contributor(s) |
Xu W, Liu T, Zhou J |
| Citation(s) |
35250958 |
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| Submission date |
Jan 03, 2022 |
| Last update date |
Mar 10, 2022 |
| Contact name |
taiping Liu |
| E-mail(s) |
liutaiping@tmmu.edu.cn
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| Organization name |
Department of Pathogenic Biology, Army Medical University (Third Military Medical University)
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| Street address |
Gaotanyan zhengjie 30, Shapingba district, Chongqing
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| City |
Chongqing |
| ZIP/Postal code |
400038 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA793845 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE192930_RAW.tar |
199.9 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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