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| Status |
Public on Mar 01, 2022 |
| Title |
Evaluation of transcriptional changes of bone marrow derived macrophages (BMDM) in response to necrotic pancreatic acinar cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate the activation and polarisation of BMDMs during the course of pancreatitis, BMDMs were co-incubated with CCK-stimulated acinar cells (+/- the serine protease inhibitor nafamostat) for 6h in an in vitro experiment. The LPS treatment served as a positive control.
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| Overall design |
We used BMDMs isolated from C57Bl/6 mice which were maintained in the presence of 20ng MCSF over 7d until they were confluent. Co-incubation was performed with freshly prepared acinar cells from C57Bl/6 mice over 6h +/- Nafamostat. BMDMs were carefully washed before RNA Isolation. Transcriptome analyses were performed using individual RNA samples.
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| Contributor(s) |
Golchert J, Homuth G, Sendler M |
| Citation(s) |
35922425 |
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| Submission date |
Dec 22, 2021 |
| Last update date |
Aug 16, 2022 |
| Contact name |
Janine Golchert |
| E-mail(s) |
janine.golchert@med.uni-greifswald.de
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| Organization name |
University Medicine Greifswald
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| Department |
Department of Functional Genomics
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| Street address |
Felix-Hausdorff-Str. 8
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| City |
Greifswald |
| ZIP/Postal code |
17475 |
| Country |
Germany |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA791688 |
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