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| Status |
Public on Feb 16, 2022 |
| Title |
Liraglutide + PYY3-36 combination therapy mimics effects of Roux-en-Y bypass on early NAFLD whilst lacking-behind in metabolic improvements. |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Background: Non-alcoholic fatty liver disease (NAFLD) is common in the general population. Treatment options for NAFLD are very limited, GLP-1 analogues, however, seem to be effective. Roux-en-Y gastric bypass (RYGB) is highly effective in terms of body weight loss and improves histologic and metabolic markers of NAFLD. We directly compared the effects of RYGB and a treatment with liraglutide and/or peptide tyrosine tyrosine 3-36 (PYY3-36) on early NAFLD. Methods: High-fat diet (HFD)-induced obese male Wistar rats were randomized into 6 treatment groups: RYGB, sham-operation (Sham), liraglutide (0.4mg/kg/day), PYY3-36 (0.1mg/kg/day), li-raglutide+PYY3-36 and saline. Following intervention and after an observation period of 4 weeks liver samples were histologically evaluated, ELISAs and RT-qPCRs +RNA sequencing were per-formed. Results: RYGB and liraglutide+PYY3-36 induced a similar body weight loss and marked histological improvements with significantly less steatosis compared to sham/saline. However, only RYGB induced significant metabolic improvements and mRNA expression changes. Con-clusions: liraglutide+PYY3-36 combination therapy mimics the positive effects of RYGB on weight reduction and on hepatic steatosis, while its effects on insulin resistance and adipose tissue dys-function (adiponectin/leptin ratio) lack behind RYGB.
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| Overall design |
Examination of changes of mRNA in different treatment groupts in livers of male Wistar rats
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| Contributor(s) |
Dischinger U, Hasinger J, Heckel T, Bischler T, Otto C, Seyfried F, Fassnacht M, Geier A, Metzner V, Herzog G |
| Citation(s) |
35160204 |
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| Submission date |
Dec 21, 2021 |
| Last update date |
Feb 18, 2022 |
| Contact name |
Tom Gräfenhan |
| E-mail(s) |
ht-seq.sysmed@uni-wuerzburg.de
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| Phone |
+49 - 931 - 31 - 80814
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| Organization name |
University of Wuerzburg
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| Department |
Core Unit SysMed
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| Lab |
Raum D15.02.045
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| Street address |
Josef-Schneider-Str. 2, Bau D15
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| City |
Würzburg |
| ZIP/Postal code |
97080 |
| Country |
Germany |
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| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
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| Samples (69)
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| Relations |
| BioProject |
PRJNA791389 |
| SRA |
SRP351984 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE192425_Raw_count_table.csv.gz |
3.9 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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