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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 04, 2009 |
| Title |
Genome-wide maps of Tbx3 binding sites in mouse ESCs |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Induced pluripotent stem (iPS) cells can be obtained through the introduction of defined factors into somatic cells. The combination of Oct4, Sox2 and Klf4 (OSK) constitutes the minimal requirement for generating iPS cells from mouse embryonic fibroblasts (MEFs). Through the genomic analyses of ESC genes that have roles in pluripotency and fusion-mediated somatic cell reprogramming, we identified Tbx3 as a transcription factor that significantly improves the quality of iPS cells. Induced-PS cells generated with OSK + Tbx3 (OSKT) are superior in both germ cell contribution to the gonads and germ-line transmission frequency. However, global gene expression profiling could not distinguish between OSK and OSKT iPS cells. Genome-wide ChIP-sequencing analysis of Tbx3 binding sites in ESCs suggests that Tbx3 regulates pluripotency-associated and reprogramming factors, in addition to sharing many common downstream regulatory targets with Oct4, Sox2, Nanog and Smad1.
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| Overall design |
ChIP-seq of Tbx3 binding in mouse ESCs
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| Contributor(s) |
Lim B, Tam W, Orlov YL |
| Citation(s) |
20139965 |
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| Submission date |
Nov 30, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Yuriy L Orlov |
| E-mail(s) |
orlovy@gis.a-star.edu.sg
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| Organization name |
Genome Institute of Singapore
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| Department |
Computational Systems Biology
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| Street address |
60 Biopolis Street
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| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
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| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
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| Samples (2) |
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| Relations |
| SRA |
SRP001585 |
| BioProject |
PRJNA120841 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE19219_RAW.tar |
88.8 Mb |
(http)(custom) |
TAR (of BED) |
SRA Run Selector |
| Processed data provided as supplementary file |
| Raw data are available in SRA |
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