|
| Status |
Public on Dec 23, 2021 |
| Title |
Genome-wide mapping of eIF3-RNA interactions in Jurkat cells using PAR-CLIP |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this study was to determine the RNAs that directly bind eukaryotic translation initiation factor eIF3 in activated and non-activated Jurkat cells.
|
| |
|
| Overall design |
Jurkat cells labeled with 4-thiouridine were treated were treated +/- phorbol 12-myristate 13-acetate (PMA) and ionomycin (IONO) for 5 hours, then crosslinked using 364 nm light, followed by isolation of RNAs crosslinked to eIF3, which were then prepared for DNA deep sequencing.
|
| |
|
| Contributor(s) |
De Silva D, Cate JH, Chin G |
| Citation(s) |
34970966 |
| |
| Submission date |
Dec 20, 2021 |
| Last update date |
Mar 24, 2022 |
| Contact name |
Jamie H D Cate |
| E-mail(s) |
j-h-doudna-cate@berkeley.edu
|
| Phone |
510-541-7235
|
| Organization name |
University of California
|
| Department |
Molecular and Cell Biology/Chemistry
|
| Street address |
2151 Berkeley Way
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720-5230 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA790960 |
Less...
More...