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Series GSE19092 Query DataSets for GSE19092
Status Public on Aug 27, 2010
Title Coordinated Progression Through Two Subtranscriptomes Underlies the Tachyzoite Cycle of Toxoplasma gondii
Organism Toxoplasma gondii
Experiment type Expression profiling by array
Summary We describe the cell cycle transcriptome of the Toxoplasma gondii that has emerged as a major genetic model for the study of Apicomplexa parasites. Two distinct transcriptional waves accompany the relatively simple binary replication of tachyzoite stage (endodyogeny) functionally separating conserved gene expression in the eukaryotic G1 phase from the lineage-specific expression that predominates in the parasite S phase and mitotic periods. This division of transcriptional focus closely mirrors the intimate relationship that has evolved between mitosis and building of the daughter parasites and invasion organelles. Promoter mechanisms appear to orchestrate the induction of gene expression in dividing tachyzoites with up to two dozen cell cycle AP2 factors likely acting within a transcriptional regulatory network to coordinate the parasite cell cycle transcriptome.
 
Overall design To characterize the cell cycle transcriptome of Toxoplasma tachyzoites, we expanded a thymidine-synchrony model to isolate sufficient RNA for microarray expression studies. The ToxoGeneChip microarray (http://ancillary.toxodb.org/docs/Array-Tutorial.html) was used to measure mRNA expression in 13 duplicate samples (R0 to R12) covering 12 hours post-synchronization and nearly two tachyzoite replication cycles.
 
Contributor(s) Behnke MS, Wootton JC, Lehmann MM, Radke JB, Lucas O, Nawas J, Sibley LD, White MW
Citation(s) 20865045
Submission date Nov 18, 2009
Last update date Sep 20, 2012
Contact name Michael Sean Behnke
E-mail(s) mike_behnke@yahoo.com
Organization name Washington University School of Medicine
Department Molecular Microbiology
Lab Dr David Sibley
Street address 660 S. Euclid Ave
City St Louis
State/province MO
ZIP/Postal code 63110
Country USA
 
Platforms (1)
GPL7186 [Tgondiia520372] Affymetrix Toxoplasma gondii Custom GeneChip (CDF: ToxoDB version 4)
Samples (28)
GSM472951 Toxoplasma gondii - CTK11/Cell Cycle - asynchronous - 1
GSM472952 Toxoplasma gondii - CTK11/Cell Cycle - asynchronous - 2
GSM472953 Toxoplasma gondii - CTK11/Cell Cycle - blocked - 1
Relations
BioProject PRJNA120513

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Supplementary file Size Download File type/resource
GSE19092_RAW.tar 26.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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