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Series GSE190847 Query DataSets for GSE190847
Status Public on Dec 14, 2021
Title Transcriptome data of B cells from patients with multiple sclerosis and healthy controls
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We analyzed the transcriptome profile of B cells from patients with relapsing-remitting multiple sclerosis (RRMS), patients with primary progressive multiple sclerosis (PPMS) and healthy individuals.
High-density Clariom D Arrays for human were used to quantify the transcript levels. This GEO entry provides the processed Clariom D microarray data from the gene-level and exon-level workflows.
 
Overall design EDTA blood samples (n=121) were taken from MS patients (n=63) and healthy controls (n=28). Up to four blood samples were collected per patient.
Seven patients were about to switch to an immune reconstitution therapy (IRT). The other RRMS patient samples were taken under therapy with alemtuzumab (n=38), natalizumab (n=29) or cladribine (n=6).
Untouched B cells were isolated from each blood sample, and total RNA was extracted, labeled and hybridized to Clariom D microarrays to obtain gene-level and exon-level expression profiles.
 
Contributor(s) Hecker M, Koczan D, Zettl UK
Citation(s) 37533036, 36405756
Submission date Dec 13, 2021
Last update date Aug 28, 2023
Contact name Michael Hecker
E-mail(s) michael.hecker@rocketmail.com
Organization name University of Rostock
Department Department of Neurology
Lab Division of Neuroimmunology
Street address Gehlsheimer Str. 20
City Rostock
ZIP/Postal code 18147
Country Germany
 
Platforms (1)
GPL23126 [Clariom_D_Human] Affymetrix Human Clariom D Assay [transcript (gene) version]
Samples (121)
GSM5732852 sample 1, healthy control 1
GSM5732853 sample 2, healthy control 2
GSM5732854 sample 3, healthy control 3
Relations
BioProject PRJNA788618

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE190847_GA_affy_B_cell_transcriptomes_MS_exon-level_Matrix.txt.gz 508.3 Mb (ftp)(http) TXT
GSE190847_RAW.tar 2.8 Gb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data are available on Series record

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