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| Status |
Public on Dec 31, 2022 |
| Title |
Single-Cell RNA sequencing reveals prophylactic Prednisone blunts cellular immunity in hemophilia B gene therapy |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Seven hemophilia B gene therapy participants were taken blood for single cell sequencing at four timepoints, and then compared the immune status of T and antigen presentation cells of theses patients according to therapy efficacy and different time points. The results showed that cellular immunity was elevated in patients with FIX:C less then 40IU/dl at week 26, glucocorticoids (GCs) effectively suppressed T cell immunity and GCs inhibited T cell immunity indirectly by effecting on APCs.
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| Overall design |
Examination of single cell mRNA profile in peripheral blood mononuclear cell from seven hemophilia B gene therapy participants at following time points：Day-7(before glucocorticoids treatment), Day 0（before AAV infusion）, Day 7(after AAV infusion) and 2 weeks after glucocorticoids withdrawal(stop GCs) .
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| |
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| Contributor(s) |
Feng X, Huiyuan L, Lei Z |
| Citation(s) |
35598604 |
| |
| Submission date |
Dec 13, 2021 |
| Last update date |
Apr 02, 2023 |
| Contact name |
Huiyuan Li |
| E-mail(s) |
lihuiyuan@ihcams.ac.cn
|
| Organization name |
Institute of Hematology & Blood Diseases Hospital
|
| Street address |
288 Nanjing Road
|
| City |
Tianjin |
| ZIP/Postal code |
300020 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (28)
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| Relations |
| BioProject |
PRJNA788436 |
| SRA |
SRP350525 |
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