|
| Status |
Public on Nov 29, 2022 |
| Title |
Survivin ChIPseq |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
ChIPseq analysis of survivin chromatin binding in activated CD4+ T cells
|
| |
|
| Overall design |
12 women, CD4 cells from PBMC isolated by positive selection on Dynabeads (cat.11331D), ConA+LPS for 72h, pooled into 4 samples. ChIPseq using anti-Survivin antibodies (2 microg/ml, 10811, SantaCruz Biotechnology). DNA purified by the EpiTect ChIP OneDay kit (Qiagen).
|
| |
|
| Contributor(s) |
Bokarewa MI, Andersson KM, Erlandsson MC, Oparina N |
| Citation(s) |
36425763 |
| |
| Submission date |
Dec 07, 2021 |
| Last update date |
Nov 29, 2022 |
| Contact name |
Maria I Bokarewa |
| E-mail(s) |
maria.bokarewa@rheuma.gu.se
|
| Organization name |
Gothenburg University
|
| Department |
Rheumatology and Inflammation Research
|
| Lab |
Bokarewa
|
| Street address |
Guldhedagatan 10A
|
| City |
Gothenburg |
| State/province |
VG |
| ZIP/Postal code |
SE41346 |
| Country |
Sweden |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (8)
|
| GSM5720377 |
Input ChIPseq sample 1, activated CD4 cells |
| GSM5720378 |
Survivin ChIPseq sample 1, activated CD4 cells |
| GSM5720379 |
Input ChIPseq sample 2, activated CD4 cells |
| GSM5720380 |
Survivin ChIPseq sample 2, activated CD4 cells |
| GSM5720381 |
Input ChIPseq sample 3, activated CD4 cells |
| GSM5720382 |
Survivin ChIPseq sample 3, activated CD4 cells |
| GSM5720383 |
Input ChIPseq sample 4, activated CD4 cells |
| GSM5720384 |
Survivin ChIPseq sample 4, activated CD4 cells |
|
| This SubSeries is part of SuperSeries: |
|
| Relations |
| BioProject |
PRJNA786829 |
| SRA |
SRP349619 |
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