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| Status |
Public on Nov 29, 2022 |
| Title |
RNAseq of CD4 T cells treated with survivivin inhibitor YM155 for 72h |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
CD4 T cells from 4 healthy women were activated with aCD3 and treated with the survivin inhibitor YM155, 10 ng/ml or control during 72 hours. The last 2 hours with upplement of IFNg. The objective was to understand the action of survivin (coded by BIRC5 gene) in CD4 T cells.
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| Overall design |
4 healthy women, paired analysis possible. CD4 cells from human PBMC isolated by positive selection on Dynabeads (cat11331D), stimulated with anti-CD3 (OKT3, Sigma-Aldrich) treated for 72h with YM155, 0 or 10nM, (Selleck Chemicals, Houston, TX) last 2 hours with addition of IFNg (50ng/ml, Peprotech, Cranbury, NJ, USA)
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| Contributor(s) |
Bokarewa MI, Andersson KM, Erlandsson MC, Oparina N |
| Citation(s) |
36425763 |
| |
| Submission date |
Dec 07, 2021 |
| Last update date |
Nov 29, 2022 |
| Contact name |
Maria I Bokarewa |
| E-mail(s) |
maria.bokarewa@rheuma.gu.se
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| Organization name |
Gothenburg University
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| Department |
Rheumatology and Inflammation Research
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| Lab |
Bokarewa
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| Street address |
Guldhedagatan 10A
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| City |
Gothenburg |
| State/province |
VG |
| ZIP/Postal code |
SE41346 |
| Country |
Sweden |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA786831 |
| SRA |
SRP349616 |
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