GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE190245 Query DataSets for GSE190245
Status Public on Jan 03, 2022
Title The profile of microRNA expression and potential role in the regulation of drug-resistant genes in cisplatin and paclitaxel resistant ovarian cancer cell lines
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Ovarian cancer is the most lethal gynecological malignancy. The high mortality results from late diag-nosis and the development of drug resistance. Drug resistance results from changes in the expression of different drug-resistance genes that may be regulated miRNA. The main aim of our study was to detect changes in miRNA expression levels in two cisplatin (CIS) and two paclitaxel (PAC) - resistant vari-ants of the A2780 drug-sensitive ovarian cancer cell line — by miRNA microarray
Overall design The human ovarian carcinoma A2780 cell line was purchased from ATCC. A2780 sublines that were resistant to CIS [A2780CR1, A2780CR2 (A2780 cisplatin resistant)], and PAC [A2780PR1 and A2780PR2 (A2780 paclitaxel resistant)] were obtained by exposing A2780 cells to the relevant drugs at gradually increasing concentrations. The final con-centrations used to select the resistant cells were 1000 ng/ml of CIS for both A2780CR1 and A2780CR2 cell lines, 300 ng/ml of PAC for A2780PR1 cell line and 1100 ng/ml of PAC for A2780PR2 cell line
Contributor(s) Kaźmierczak D, Ruciński M, Januchowski R
Citation(s) 35008952, 35628654
Submission date Dec 06, 2021
Last update date Jun 21, 2022
Contact name Marcin Rucinski
Organization name Poznan University of Medical Science
Department Histology and Embriology
Street address Swiecicki 6
City Poznan
ZIP/Postal code 60-781
Country Poland
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (15)
GSM5718614 A2780 cell line, biological rep 1
GSM5718615 A2780 cell line, biological rep 2
GSM5718616 A2780 cell line, biological rep 3
BioProject PRJNA786532

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE190245_RAW.tar 12.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap