 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 03, 2022 |
| Title |
The profile of microRNA expression and potential role in the regulation of drug-resistant genes in cisplatin and paclitaxel resistant ovarian cancer cell lines |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Ovarian cancer is the most lethal gynecological malignancy. The high mortality results from late diag-nosis and the development of drug resistance. Drug resistance results from changes in the expression of different drug-resistance genes that may be regulated miRNA. The main aim of our study was to detect changes in miRNA expression levels in two cisplatin (CIS) and two paclitaxel (PAC) - resistant vari-ants of the A2780 drug-sensitive ovarian cancer cell line — by miRNA microarray
|
| |
|
| Overall design |
The human ovarian carcinoma A2780 cell line was purchased from ATCC. A2780 sublines that were resistant to CIS [A2780CR1, A2780CR2 (A2780 cisplatin resistant)], and PAC [A2780PR1 and A2780PR2 (A2780 paclitaxel resistant)] were obtained by exposing A2780 cells to the relevant drugs at gradually increasing concentrations. The final con-centrations used to select the resistant cells were 1000 ng/ml of CIS for both A2780CR1 and A2780CR2 cell lines, 300 ng/ml of PAC for A2780PR1 cell line and 1100 ng/ml of PAC for A2780PR2 cell line
|
| |
|
| Contributor(s) |
Kaźmierczak D, Ruciński M, Januchowski R |
| Citation(s) |
35008952, 35628654 |
| |
| Submission date |
Dec 06, 2021 |
| Last update date |
Jun 21, 2022 |
| Contact name |
Marcin Rucinski |
| E-mail(s) |
marcinruc@ump.edu.pl
|
| Organization name |
Poznan University of Medical Science
|
| Department |
Histology and Embriology
|
| Street address |
Swiecicki 6
|
| City |
Poznan |
| ZIP/Postal code |
60-781 |
| Country |
Poland |
| |
|
| Platforms (1) |
| GPL19117 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array |
|
| Samples (15)
|
| GSM5718617 |
A2780 cisplatin resistant 1, biological rep 1 |
| GSM5718618 |
A2780 cisplatin resistant 1, biological rep 2 |
| GSM5718619 |
A2780 cisplatin resistant 1, biological rep 3 |
| GSM5718620 |
A2780 cisplatin resistant 2, biological rep 1 |
| GSM5718621 |
A2780 cisplatin resistant 2, biological rep 2 |
| GSM5718622 |
A2780 cisplatin resistant 2, biological rep 3 |
| GSM5718623 |
A2780 paclitaxel resistant 1, biological rep 1 |
| GSM5718624 |
A2780 paclitaxel resistant 1, biological rep 2 |
| GSM5718625 |
A2780 paclitaxel resistant 1, biological rep 3 |
| GSM5718626 |
A2780 paclitaxel resistant 2, biological rep 1 |
| GSM5718627 |
A2780 paclitaxel resistant 2, biological rep 2 |
| GSM5718628 |
A2780 paclitaxel resistant 2, biological rep 3 |
|
| Relations |
| BioProject |
PRJNA786532 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE190245_RAW.tar |
12.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...