|
| Status |
Public on Dec 25, 2021 |
| Title |
Profiling the transcriptional and translational response to BRAF targeted therapy in melanoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We examined the transcriptional and translational response to BRAF targeted therapy in BRAF mutant melanoma by applying RNA-seq to polysome profiling, a method that isolates mRNA bound to actively translating ribosomes. By applying a published data analysis pipeline, anota2seq, we determined differentially expressed genes in total RNA and polysome-bound RNAseq datasets. We performed the analysis on two treatment timepoints, allowing determination of changes in expression that occur across different treatment times. These analyses identified extensive translational reprogramming during the BRAF targeted therapy response.
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| |
|
| Overall design |
Examination of total mRNA and polysome-bound mRNA in BRAF mutant melanoma cells treated with the BRAF inhibitor vemurafenib (0, 24h, 40h).
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| |
|
| Contributor(s) |
Smith LK |
| Citation(s) |
35232962 |
| |
| Submission date |
Dec 02, 2021 |
| Last update date |
Mar 30, 2022 |
| Contact name |
Lorey K Smith |
| E-mail(s) |
lorey.smith@petermac.org
|
| Organization name |
Peter MacCallum Cancer Centre
|
| Street address |
4/30 Mater St
|
| City |
Melbourne |
| State/province |
VIC |
| ZIP/Postal code |
3066 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (22)
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| Relations |
| BioProject |
PRJNA791750 |
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