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Status |
Public on Jul 20, 2022 |
Title |
tsRNAs Are Potential Biomarkers of Acute Rejection in Vascularized Composite Allotransplantation |
Organism |
Rattus norvegicus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Purpose: to explore the function and mechanism of acute rejection in vascularized composite allotransplantation Method: tRF & tiRNA profiles of acute rejection in vascularized composite allotransplantation were generated by deep sequencing, using Illumina NextSeq 500. qRT–PCR validation was performed using SYBR Green assays. Results:Sequencing was used to screen expression profiles and predict target genes. Compared with the control group, there were significant differences in the expression levels of 16 tRFs & tiRNAs, including 5 up-regulated target genes and 11 down-regulated target genes. Bioinformatics analysis and RT-qPCR revealed potential up regulation of tRFs & tiRNAs(tiRNA-1-33-Gly-GCC-1, tiRNA-1-34-Glu-CTC-1, tRF-1-32-Gly-GCC-2-M2) and down regulation of tRFs & tiRNAs (tRF-59:75-Gln-CTG-2-M2, tRF-59:76-Val-AAC-1-M2). Conclusion: In conclusion, it is speculated that tiRNA-1-34-Glu-CTC-1 plays an important role in VCA induced acute rejection by regulating CACNA1D gene in MAPK signaling pathway. This provides a new insight into the mechanism and therapeutic targets of acute rejection.
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Overall design |
tRF & tiRNA profiles of normal skin and UV damage skin mice
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Contributor(s) |
Fang Y, Wang S |
Citation missing |
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Submission date |
Dec 02, 2021 |
Last update date |
Jul 24, 2022 |
Contact name |
yuan fang |
E-mail(s) |
jingji5996@gmail.com
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Phone |
15273191040
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Organization name |
The Third Xiangya Hospital of Central South Uniiversity
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Street address |
138 TongZiPo road
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City |
chang sha |
ZIP/Postal code |
410013 |
Country |
China |
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Platforms (1) |
GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
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Samples (6)
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Relations |
BioProject |
PRJNA785574 |
SRA |
SRP348911 |