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| Status |
Public on Jan 21, 2010 |
| Title |
Effects of IGF 1 on primary breast fibroblasts (normal and carcinoma associated) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To characterize the effects of IGF 1 on primary breast fibroblasts, we cultured pre-starved primary breast fibroblasts (normal and carcinoma associated) from three patients with or without 50ng/ml IGF 1 for 24 h. We then profiled gene expression changes using Human Exonic Evidence Based Oligonucleotide (HEEBO) microarrays. After stimulation, total RNA was extracted and amplified using a modified Eberwine procedure. The amplified RNA was labeled with the fluorescent dye Cy5 and pooled with Cy3 labeled reference RNA, and then the pooled RNA was hybridized onto HEEBO microarrays. After hybridization and washing, arrays were scanned on a fluorescent microscope scanner.
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| Overall design |
An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract.
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| Contributor(s) |
Rajski M |
| Citation(s) |
20051100 |
| |
| Submission date |
Nov 09, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Michal Rajski |
| E-mail(s) |
rajski.michal@gmail.com
|
| Organization name |
University of Zurich
|
| Department |
Institute of Physiology
|
| Lab |
23K86
|
| Street address |
Winterthurerstrasse 190
|
| City |
Zurich |
| ZIP/Postal code |
CH - 8057 |
| Country |
Switzerland |
| |
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| Platforms (1) |
| GPL9551 |
SMD Print_1451 Homo sapiens |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE18955 |
Effects of IGF 1 on primary breast fibroblasts, MCF-7 breast cancer cells and CCL-171 fibroblasts |
|
| Relations |
| BioProject |
PRJNA123879 |
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