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Status |
Public on Sep 14, 2022 |
Title |
Identification of genes associated with bortezomib-based treatment in multiple myeloma patients through bone marrow single-cell RNA-Seq |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
In this study, we performed scRNA-seq analyses of bone marrow mononuclear cells (BM-MNCs) in patients with MM receiving bortezomib-based treatments to investigate following questions; (i) the compositional differences in BM-MNCs between normal control and MM patients, and between good and poor responder groups, and (ii) the genes and the cell-to-cell communication networks associated with the treatment responsiveness.
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Overall design |
For scRNA-seq, we used baseline bone marrow aspirates (treatment-naïve) from 18 MMs, who treated with bortezomib-melphalan-prednisolone. The single-cell library preparation relied on a commercially available droplet method using the 10x Genomic Chromium System and Single Cell 3' v3 Reagent Kit according to the manufacturer’s protocol.
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Contributor(s) |
Jung S, Park S, Lee S, Chung Y, Min C |
Citation(s) |
36380017 |
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Submission date |
Nov 23, 2021 |
Last update date |
Nov 17, 2022 |
Contact name |
SeungHyun Jung |
E-mail(s) |
hyun@catholic.ac.kr
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Phone |
82-2-2258-7509
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Organization name |
Catholic University of Korea
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Department |
Department of Biochemistry
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Street address |
catholic university, medical college, banpo-dong
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City |
seoul |
State/province |
seoul |
ZIP/Postal code |
06591 |
Country |
South Korea |
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Platforms (1) |
GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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Samples (18)
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Relations |
BioProject |
PRJNA783047 |
SRA |
SRP347513 |