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| Status |
Public on Jan 12, 2024 |
| Title |
Cortical miR-709 links glutamatergic signaling to NREM sleep EEG slow waves in an activity-dependent manner [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Micro-RNAs (miRNAs) are key, post-transcriptional regulators of gene expression that have been implicated in a plethora of biological processes. Their role in sleep, however, has received little attention. In the current experiment, we investigated the effects of in vitro miR-709 inhibition on the transcriptome of primary cortical neurons.
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| Overall design |
Eighteen primary cortical neuronal cultures from C57BL6/J mice were distributed in 3 groups, each one following a different treatment, namely miR-709 inhibition (i-miR-709, n=6), scrambled control (scr, n=6) or ddH2O control (ctrl, n=6), and their transcriptome was subsequently assessed by RNA-sequencing.
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| Contributor(s) |
Kompotis K, Mang G, Franken P, Wigger L |
| Citation(s) |
38207077 |
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| Submission date |
Nov 22, 2021 |
| Last update date |
Jan 13, 2024 |
| Contact name |
Leonore Wigger |
| E-mail(s) |
leonore.wigger@sib.swiss
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| Organization name |
Swiss Institute of Bioinformatics
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| Lab |
Vital-IT
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| Street address |
Amphipôle Building
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| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (18)
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| GSM5699366 |
c15B, control, batch 6 |
| GSM5699367 |
c28A, control, batch 1 |
| GSM5699368 |
c28B, control, batch 2 |
| GSM5699369 |
m01A, inhibited, batch 3 |
| GSM5699370 |
m01B, inhibited, batch 4 |
| GSM5699371 |
m15A, inhibited, batch 5 |
| GSM5699372 |
m15B, inhibited, batch 6 |
| GSM5699373 |
m28A, inhibited, batch 1 |
| GSM5699374 |
m28B, inhibited, batch 2 |
| GSM5699375 |
s01A, scrambled, batch 3 |
| GSM5699376 |
s01B, scrambled, batch 4 |
| GSM5699377 |
s15A, scrambled, batch 5 |
| GSM5699378 |
s15B, scrambled, batch 6 |
| GSM5699379 |
s28A, scrambled, batch 1 |
| GSM5699380 |
s28B, scrambled, batch 2 |
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| This SubSeries is part of SuperSeries: |
| GSE189353 |
Cortical miR-709 links glutamatergic signaling to NREM sleep EEG slow waves in an activity-dependent manner |
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| Relations |
| BioProject |
PRJNA782602 |
| SRA |
SRP347235 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE189335_RAW.tar |
3.1 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE189335_genes.htseq.normalized.txt.gz |
2.6 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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