 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 10, 2022 |
| Title |
Proteomic and single-cell transcriptomic dissection of human plasmacytoid dendritic cell response to influenza virus |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Plasmacytoid dendritic cells (pDCs) represent a rare innate immune subset uniquely endowed with the capacity to produce substantial amounts of type-I interferons (IFN-I). This function of pDCs is critical for effective antiviral defenses and has been implicated in autoimmunity. While IFN-I and select cytokines have been recognized as pDC secreted products, a comprehensive agnostic profiling of the pDC secretome in response to a physiologic stimulus has not been reported. We applied LC-MS/MS to catalogue the repertoire of proteins secreted by pDCs in response to challenge with live influenza H1N1. Additionally, using single-cell RNA-seq [scRNA-seq], we perform multidimensional analyses of pDC transcriptional diversification following stimulation. Our data reveal an abundance of protein species released by pDCs in addition to IFN-I, and evidence highly specialized roles within the pDC population ranging from dedicated cytokine super-producers to cells with APC-like functions. Moreover, dynamic expression of transcription factors and surface markers characterize activated pDC fates.
|
| |
|
| Overall design |
pDCs were stimulated with flu virus and examined at 0, 6 and 24 hours to see their temporal response
|
| |
|
| Contributor(s) |
Ghanem MH, Shih AJ, Khalili H, Werth E, Chakrabarty JK, Brown LM, Simpfendorfer KR, Gregersen PK |
| Citation(s) |
35401570 |
| |
| Submission date |
Nov 18, 2021 |
| Last update date |
Apr 19, 2022 |
| Contact name |
Andrew Shih |
| E-mail(s) |
ashih@northwell.edu
|
| Phone |
516-562-2960
|
| Organization name |
Feinstein Institute - Northwell Health
|
| Street address |
350 Community Dirive
|
| City |
Mahasset |
| State/province |
NY |
| ZIP/Postal code |
11030 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (3) |
|
| Relations |
| BioProject |
PRJNA781587 |
| SRA |
SRP346769 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE189120_RAW.tar |
106.2 Mb |
(http)(custom) |
TAR (of H5) |
| GSE189120_pDC_single_total_meta_data_update.csv.gz |
550.0 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
|
|
|
|
 |
