 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jul 13, 2022 |
| Title |
MCF-7 Cell Derived Soluble Factors Define Growth of Vascular and Lymphatic Endothelial Cells: Modulatory Role of miR193a |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Angiogenesis and Lymphangiogenesis plays a key role in promoting tumor growth and breast cancer progression as well as metastasis. Active angiogenic process within the tumor tissue is known to promote cancer/tumor growth via nutrient supply, moreover, soluble molecules secreted by lymphatic endothelial cells (ECs) promotes tumor growth. Hence is feasible that paracrine factors generated by breast cancer cells play a role in regulating tumor neovascularization by lymphatic and vascular ECs. Here using cultured VECs and LECs assessed the paracrine impact of MCF-7 breast cancer derived soluble factors on LEC and VEC growth (cell number), gene expression (micro array) and angiogenic proteins (protein array). Moreover, we explored the modulatory actions of miR193 on LEC and VEC growth driven by MCF-7 derived conditioned medium (MCF7-CM). We demonstrate that MCF7-CM differentially modulates VEC and LEC growth, i.e. induces VEC growth and inhibits LEC growth. Moreover, the growth actions of MCF7-CM were accompanied with differential modulation VEC and LEC growth regulatory genes, that were further altered by miR193 a pretreatment of MCF7 cells. Our findings provide in-depth analysis of MCF7-CM altered genes relevant for angiogenesis and lymphogenesis. The fact that vascular capillaries support growth and lymphatic capillaries play a role counteracting cancer growth (kill cancer cells), together with our observation in VECs and LECs, suggests that MCF-7 breast cancer cells differentially modulate vascular and lymphatic growth to facilitate their survival and growth. Since miR193a abrogates the effects of MCF7-CM, it may be an effective therapeutic tool against breast cancer.
|
| |
|
| Overall design |
Proliferation, migration and angiogenesis arrays were performed to investigate the effects of the MCF7-CM on VECs and LECs.Microarray analysis was conducted to determine the genes involved in the differentilly effects on both endothelial cells. Additionally the cells were exposed to consitioned medium collected from MCF-7 cells pre-treated with and without E2 or pre-transfected with miR193a to determine possible effects of the sex hormone and estradiol sensitive miRs. CM (conditioned medium).
|
| |
|
| Contributor(s) |
Dubey RK, Azzarito G, Kurmann LM, Dubey RK |
| Citation(s) |
35806196, 36230929, 36766731 |
| |
| Submission date |
Nov 18, 2021 |
| Last update date |
Mar 02, 2023 |
| Contact name |
Giovanna Azzarito |
| Organization name |
University Hospital Zurich
|
| Department |
Reproductive endocrinology
|
| Street address |
Wagistrasse 14
|
| City |
Schlieren |
| ZIP/Postal code |
8952 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
|
| Samples (8)
|
| GSM5694351 |
lymphatic endothelial cells+MCF-7 epithelial cells, mimic CTR |
| GSM5694352 |
lymphatic endothelial cells+MCF-7epithelial cells, miRNA193a |
| GSM5694353 |
vascular endothelial cells, CM by MCF-7 pre-transfected with mimic CTR |
| GSM5694354 |
vascular endothelial cells, CM by MCF-7 pre-transfected with miRNA193a |
| GSM5694355 |
vascular endothelial cells, medium without MCF-7 cells |
| GSM5694356 |
vascular endothelial cells, CM by MCF-7 cells |
| GSM5694357 |
lymphatic endothelial cells, medium without MCF-7 cells |
| GSM5694358 |
lymphatic endothelial cells, CM by MCF-7 cells |
|
| Relations |
| BioProject |
PRJNA781497 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE189084_HUVEC_CM_CTR_vs_miR193.xlsx |
1.9 Mb |
(ftp)(http) |
XLSX |
| GSE189084_HUVEC_CM_vs._CTR.xlsx |
1.9 Mb |
(ftp)(http) |
XLSX |
| GSE189084_LEC_CM_vs._CTR.xlsx |
1.9 Mb |
(ftp)(http) |
XLSX |
| GSE189084_LECs_spheroids_miR193a_vs_CTR_.xlsx |
1.9 Mb |
(ftp)(http) |
XLSX |
| GSE189084_RAW.tar |
25.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...