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| Status |
Public on Mar 14, 2022 |
| Title |
Study of ebola virus VP35 protein on gene expression in HepG2 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The overall effect of VP35 on host gene expression were investigated by the differentially expressed genes (DEG) analysis in cells transfected with or without Flag-VP35
Gene expression in HepG2 cells transfected with VP35 or vector was analyzed with a gene expression microarray
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| Overall design |
HepG2 cells were transfected with the plasmids Flag-VP35 or Flag, and each group has three independent replicates. Forty-eight hours later, total RNA from six samples was extracted using the RNeasy mini kit (QIAgen, USA) according to the manufacturer’s protocol. After first-strand cDNA synthesis with Poly-dT primer and second-strand cDNA synthesis, the cDNA was subjected to Gene Expression Microarray analysis following the manufacturer’s protocol. The chips were scanned with a GeneChip Scanner G2565CA (Agilent).
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| |
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| Contributor(s) |
Zhu L |
| Citation(s) |
35474062 |
| |
| Submission date |
Nov 11, 2021 |
| Last update date |
May 05, 2022 |
| Contact name |
Lin Zhu |
| E-mail(s) |
zhul627@gmail.com
|
| Organization name |
Academy of military medical sciences AMS PLA
|
| Street address |
Taiping 27 road
|
| City |
beijing |
| ZIP/Postal code |
100850 |
| Country |
China |
| |
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| Platforms (1) |
| GPL21185 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Probe Name Version] |
|
| Samples (6)
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| GSM5687334 |
HepG2 cells_transfection Flag-VP35_48 hours_rep1 |
| GSM5687335 |
HepG2 cells_transfection Flag-VP35_48 hours_rep2 |
| GSM5687336 |
HepG2 cells_transfection Flag-VP35_48 hours_rep3 |
| GSM5687337 |
HepG2 cells_transfection Flag-Vector_48 hours_rep1 |
| GSM5687338 |
HepG2 cells_transfection Flag-Vector_48 hours_rep2 |
| GSM5687339 |
HepG2 cells_transfection Flag-Vector_48 hours_rep3 |
|
| Relations |
| BioProject |
PRJNA779721 |
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