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| Status |
Public on Mar 06, 2023 |
| Title |
Transcriptome and translatome profiling of warm temperature (35°C -> 38.5°C) treated mouse embryonic fibroblast cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
To see a possible effect of a physiological warm temperature shift (WTS, 35°C to 38.5°C) on Per2 transcription and translation in mammalian cells, we performed RNA-seq and Ribo-seq using dexamethasone-synchronized mouse embryonic fibroblast cells with or without WTS treatment at a circadain rising phase of Per2.
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| Overall design |
We used Dex-synchronized MEF cells treated with or without 2-h WTS. WTS was applied 24 h after Dex synchronization (i.e., circadian rising phase of Per2). Three independent experiments were performed.
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| Contributor(s) |
Masao D, Shintaro I, Takahito M |
| Citation(s) |
36882059 |
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| Submission date |
Nov 10, 2021 |
| Last update date |
Jun 10, 2023 |
| Contact name |
Masao Doi |
| E-mail(s) |
doimasao@pharm.kyoto-u.ac.jp
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| Phone |
81-75753-9555
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| Organization name |
Kyoto University, Graduate School of Pharmaceutical Sciences
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| Department |
Department of Systems Biology
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| Street address |
46-29 Yoshida-Shimo-Adachi-cho, Sakyo-ku
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| City |
Kyoto |
| ZIP/Postal code |
6068501 |
| Country |
Japan |
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| Platforms (2) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA779283 |
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