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| Status |
Public on Jan 11, 2010 |
| Title |
Chronic Active B Cell Receptor Signaling in Diffuse Large B Cell Lymphoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To determine gene expression of DLBCL upon NF-kB inhibition or knockdown of BCR signaling components.
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| Overall design |
Total RNA (Trizol reagent; Invitrogen, Carlsbad, CA) was prepared from 8 HBL-1 samples following incubation with 25 µmol/L MLN120B (Millennium Pharmaceuticals, Cambridge, MA) for 2, 3, 4, 6, 8, 12, 16, and 24 hours. In addition, 16 HBL-1 samples were infected with retroviral vectors expressing various shRNAs (CARD11, SYK, BTK, CD79A) in a doxycycline-inducible fashion, selected with puromycin, treated with doxycycline for 24 or 48 hr and then harvested for total RNA. Uninduced cultures were prepared in parallel as control samples. Biological replicates were performed for the CARD11, BTK, and CD79A samples.
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| Citation(s) |
20054396 |
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| Submission date |
Oct 30, 2009 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Louis M. Staudt |
| E-mail(s) |
lstaudt@mail.nih.gov
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| Phone |
301-402-1892
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| Organization name |
National Cancer Institute
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| Department |
Lymphoid Malignancies Branch
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| Lab |
Louis M Staudt
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| Street address |
9000 Rockville Pike, Bldg 10, Rm 4N114
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (24)
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| GSM466597 |
MLN120B Treated HBL-1 - 2 hrs - mAdbID:95899 |
| GSM466598 |
MLN120B Treated HBL-1 - 4 hrs - mAdbID:95900 |
| GSM466599 |
MLN120B Treated HBL-1 - 8 hrs - mAdbID:95901 |
| GSM466600 |
MLN120B Treated HBL-1 - 16 hrs - mAdbID:95902 |
| GSM466601 |
BTK transduced HBL-1 - 24 hrs - repeat 2 - mAdbID:96141 |
| GSM466602 |
SYK transduced HBL-1 - 24 hrs - mAdbID:96142 |
| GSM466603 |
CD79A transduced HBL-1 - 24 hrs - repeat 3 - mAdbID:96143 |
| GSM466604 |
BTK transduced HBL-1 - 48 hrs - repeat 2 - mAdbID:96975 |
| GSM466605 |
SYK transduced HBL-1 - 48 hrs - mAdbID:96976 |
| GSM466606 |
CD79A transduced HBL-1 - 48 hrs - repeat 3 - mAdbID:96977 |
| GSM466607 |
CD79A transduced HBL-1 - 24 hrs - repeat 2 - mAdbID:98863 |
| GSM466608 |
CD79A transduced HBL-1 - 48 hrs - repeat 2 - mAdbID:98864 |
| GSM466609 |
CARD11 transduced HBL-1 - 24 hrs - repeat 2 - mAdbID:98865 |
| GSM466610 |
CARD11 transduced HBL-1 - 48 hrs - repeat 2 - mAdbID:98866 |
| GSM466611 |
BTK transduced HBL-1 - 24 hrs - repeat 1 - mAdbID:98869 |
| GSM466612 |
BTK transduced HBL-1 - 48 hrs - repeat 1 - mAdbID:98870 |
| GSM466613 |
MLN120B Treated HBL-1 - 3 hrs - mAdbID:99297 |
| GSM466614 |
MLN120B Treated HBL-1 - 6 hrs - mAdbID:99298 |
| GSM466615 |
MLN120B Treated HBL-1 - 12 hrs - mAdbID:99299 |
| GSM466616 |
MLN120B Treated HBL-1 - 24 hrs - mAdbID:99300 |
| GSM466617 |
CARD11 transduced HBL-1 - 24 hrs - repeat 1 - mAdbID:99356 |
| GSM466618 |
CARD11 transduced HBL-1 - 48 hrs - repeat 1 - mAdbID:99357 |
| GSM466619 |
CD79A transduced HBL-1 - 24 hrs - repeat 1 - mAdbID:99358 |
| GSM466620 |
CD79A transduced HBL-1 - 48 hrs - repeat 1 - mAdbID:99359 |
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| Relations |
| BioProject |
PRJNA121241 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE18817_RAW.tar |
355.6 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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