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Series GSE18817 Query DataSets for GSE18817
Status Public on Jan 11, 2010
Title Chronic Active B Cell Receptor Signaling in Diffuse Large B Cell Lymphoma
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To determine gene expression of DLBCL upon NF-kB inhibition or knockdown of BCR signaling components.
 
Overall design Total RNA (Trizol reagent; Invitrogen, Carlsbad, CA) was prepared from 8 HBL-1 samples following incubation with 25 ┬Ámol/L MLN120B (Millennium Pharmaceuticals, Cambridge, MA) for 2, 3, 4, 6, 8, 12, 16, and 24 hours. In addition, 16 HBL-1 samples were infected with retroviral vectors expressing various shRNAs (CARD11, SYK, BTK, CD79A) in a doxycycline-inducible fashion, selected with puromycin, treated with doxycycline for 24 or 48 hr and then harvested for total RNA. Uninduced cultures were prepared in parallel as control samples. Biological replicates were performed for the CARD11, BTK, and CD79A samples.
 
Citation(s) 20054396
Submission date Oct 30, 2009
Last update date Feb 22, 2018
Contact name Louis M. Staudt
E-mail(s) lstaudt@mail.nih.gov
Phone 301-402-1892
Organization name National Cancer Institute
Department Lymphoid Malignancies Branch
Lab Louis M Staudt
Street address 9000 Rockville Pike, Bldg 10, Rm 4N114
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (24)
GSM466597 MLN120B Treated HBL-1 - 2 hrs - mAdbID:95899
GSM466598 MLN120B Treated HBL-1 - 4 hrs - mAdbID:95900
GSM466599 MLN120B Treated HBL-1 - 8 hrs - mAdbID:95901
Relations
BioProject PRJNA121241

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE18817_RAW.tar 355.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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