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| Status |
Public on Jul 29, 2022 |
| Title |
Transcriptional effects of catalytic mTOR inhibiton among LPS-stimulated primary human monocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To better understand the role of mTOR in regulation of the monocyte response to LPS, we obtained mRNA-seq profiles of primary human monocytes stimulated ex vivo through TLR4, with and without pretreatment with a catalytic mTOR inhibitor (mTORi)
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| Overall design |
We obtained primary human monocytes from six independent donors and stimulated with LPS (1 ng/mL) or vehicle for 6h following pretreatment with an mTORi (AZD2014 at 5μM) or DMSO for 6h ex vivo, prior to extraction of RNA and library prep.
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| |
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| Contributor(s) |
D'Aquila RT, Taylor HE, Calantone N |
| Citation(s) |
36028574 |
| |
| Submission date |
Nov 03, 2021 |
| Last update date |
Aug 31, 2022 |
| Contact name |
Richard Thomas D'Aquila |
| E-mail(s) |
richard.daquila@northwestern.edu
|
| Organization name |
Northwestern University Feinberg School of Medicine
|
| Department |
Medicine (Infectious Diseases)
|
| Street address |
303 E Superior St Lurie Research 9-220
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA777550 |
| SRA |
SRP344362 |
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