|
| Status |
Public on Apr 01, 2022 |
| Title |
Small RNA profiling of differentiated and de-differentiated vascular smooth muscle cell |
| Organism |
Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
We report the application of small RNA sequencing for high-throughput profiling of small RNA under 75 bp in vascular smooth muscle cell. By a reading depth of 30M and single stranded sequencing, we generated the small RNA signature on differentiated and de-differentiated vascular smooth muscle cell induced by PDGF-BB and H3K4me2 editing. We found that PDGF-BB and H3K4me2 editing induced de-differentiation modulated miRNA profile significantly, which was demonstrated at least in part responsible for modulated vascular smooth muscle cell phenotype.
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| |
|
| Overall design |
Profiling of differentiated and PDGF-BB and H3K4me2 edited de-differentiated vascular smooth muscle cell
|
| |
|
| Contributor(s) |
Du M, Espinosa C, Liu M, Mahan S, Handen A, Chan SY, Gomez D |
| Citation(s) |
35465051 |
| |
| Submission date |
Nov 02, 2021 |
| Last update date |
Apr 27, 2022 |
| Contact name |
Delphine Gomez |
| E-mail(s) |
gomezd@pitt.edu
|
| Organization name |
University of Pittsburgh
|
| Department |
Department of Medicine, Division of Cardiology
|
| Street address |
200 Lothrop St
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15261 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
|
| Samples (9)
|
| GSM5664564 |
518_1: Differentiated vascular smooth muscle cell_rep1 |
| GSM5664565 |
518_2: Differentiated vascular smooth muscle cell_rep2 |
| GSM5664566 |
518_3: Differentiated vascular smooth muscle cell_rep3 |
| GSM5664567 |
BB_1: PDGF-BB induced de-differentiated vascular smooth muscle cell_rep1 |
| GSM5664568 |
BB_2: PDGF-BB induced de-differentiated vascular smooth muscle cell_rep2 |
| GSM5664569 |
BB_3: PDGF-BB induced de-differentiated vascular smooth muscle cell_rep3 |
| GSM5664570 |
1A_1: H3K4me2 edited de-differentiated vascular smooth muscle cell_rep1 |
| GSM5664571 |
1A_2: H3K4me2 edited de-differentiated vascular smooth muscle cell_rep2 |
| GSM5664572 |
1A_3: H3K4me2 edited de-differentiated vascular smooth muscle cell_rep3 |
|
| Relations |
| BioProject |
PRJNA777063 |
| SRA |
SRP344179 |
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