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| Status |
Public on Nov 03, 2021 |
| Title |
α6β2* nAChR Antagonist TxIB Changed Gene Expression in Zebrafish (Danio rerio) brain Induced by Alcohol Withdrawal |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Background and Purpose: Alcohol use disorder (AUD) is a serious public health issue and affects the lives of numerous people. Previous studies have shown a link between nicotinic acetylcholine receptors (nAChR) and alcohol addiction. However, the role of α6β2* nAChR in alcohol addiction remains obscure and whether α6β2* nAChR can be used as a potential drug target for alcohol withdrawal need to be studied. Methods: Zebrafish (Danio rerio) were exposed to 0.2% alcohol for 14 days followed by 7 days of repeated withdrawal and then retro-orbitally injected with α-conotoxin TxIB (a selective α6β2* nAChR antagonist). RNA-sequencing (RNA-seq) and subsequent bioinformatics analysis were applied to explore the potential network regulation by blocking α6β2* nAChR using TxIB after alcohol withdrawal. Results: RNA sequencing of zebrafish brain indicated a total of 657 genes showed aberrant expression and among which 225 were reversed after TxIB injection. These reversed genes were significantly enriched in cardiac muscle contraction and calcium signaling pathways and the gene expression profile was further validated by RT-PCR. Conclusion: Our finding suggests α6β2* nAChR antagonists have the potential to modulate alcohol withdrawal.
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| Overall design |
Three groups were divided into normal, alcohol-withdrawal, and TxIB. Each group has 3 biological replicates.
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| Contributor(s) |
Mao K, Li X, Chen Z, Dong X, Zhangsun D, Zhu X, Luo S |
| Citation(s) |
35177988 |
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Mao et al. Frontiers in Pharmacology. 01 February 2022. https://doi.org/10.3389/fphar.2022.802917
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| |
| Submission date |
Nov 01, 2021 |
| Last update date |
Feb 23, 2022 |
| Contact name |
Kailin Mao |
| E-mail(s) |
maokl@hainanu.edu.cn
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| Organization name |
Hainan University
|
| Department |
School of Life Sciences
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| Lab |
Key Laboratoryr of Topical Biological Resources of Ministry of Education
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| Street address |
Hai Dian Campus of Hainan University, No.58 of Ren Min Road, Mei Lan District, Haikou, Hainan Province,China
|
| City |
Haikou |
| State/province |
Hai Nan |
| ZIP/Postal code |
570228 |
| Country |
China |
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| Platforms (1) |
| GPL24995 |
Illumina NovaSeq 6000 (Danio rerio) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA776830 |
| SRA |
SRP344020 |
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